Literature DB >> 31407052

ArdB Protective Activity for Unmodified λ Phage Against EcoKI Restriction Decreases in UV-Treated Escherichia coli.

Vladimir P Balabanov1, Anna A Kudryavtseva2, Olga E Melkina1, Klara S Pustovoit1, Svetlana A Khrulnova1,3, Gennadii B Zavilgelsky1.   

Abstract

Anti-restriction proteins ArdB/KlcA specifically inhibit restriction (endonuclease) activity of restriction-modification (RM) type I systems. Molecular mechanisms of ArdB/KlcA-based anti-restriction remain unknown. In this study, we quantitate effects of ArdB on protection of unmodified λ phage DNA from EcoKI restriction. After UV irradiations, which produce significant amounts of unmodified chromosomal DNA in Escherichia coli K12 cells, the protective activity of ArdB decreases. Unlike ArdB, DNA-mimicking protein Ocr retains its ability to protect the unmodified λ phage regardless of UV dose. We hypothesize that the observed decrease in ArdB protective activity in UV-treated cells is due to its binding to unmodified chromosomal DNA, which decreases effective concentrations of free ArdB molecules available for λ phage protection against type I restriction enzymes.

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Year:  2019        PMID： 31407052     DOI： 10.1007/s00284-019-01755-z

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  17 in total

1.  Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes.

Authors:  S Makovets; V A Doronina; N E Murray
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

2.  The proteolytic control of restriction activity in Escherichia coli K-12.

Authors:  V A Doronina; N E Murray
Journal:  Mol Microbiol       Date:  2001-01       Impact factor: 3.501

3.  Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA.

Authors:  M D Walkinshaw; P Taylor; S S Sturrock; C Atanasiu; T Berge; R M Henderson; J M Edwardson; D T F Dryden
Journal:  Mol Cell       Date:  2002-01       Impact factor: 17.970

4.  Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme.

Authors:  C Atanasiu; T-J Su; S S Sturrock; D T F Dryden
Journal:  Nucleic Acids Res       Date:  2002-09-15       Impact factor: 16.971

5.  Control of the endonuclease activity of type I restriction-modification systems is required to maintain chromosome integrity following homologous recombination.

Authors:  Garry W Blakely; Noreen E Murray
Journal:  Mol Microbiol       Date:  2006-05       Impact factor: 3.501

6.  Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli.

Authors:  Ivana Ivancić-Bacće; Ignacija Vlasić; Gordana Cogelja-Cajo; Krunoslav Brcić-Kostić; Erika Salaj-Smic
Journal:  Genetics       Date:  2006-10-08       Impact factor: 4.562

7.  [Antimodification activity of the ArdA and Ocr proteins].

Authors:  G V Zavil'gel'skiĭ; V Iu Kotova; S M Rastorguev
Journal:  Genetika       Date:  2011-02

8.  [Comparative analysis of antirestriction activity of R64 ArdA and ArdB proteins].

Authors:  V P Balabanov; K S Pustovoĭt; G B Zavil'gel'skiĭ
Journal:  Mol Biol (Mosk)       Date:  2012 Mar-Apr

9.  Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.

Authors:  K L Guan; J E Dixon
Journal:  Anal Biochem       Date:  1991-02-01       Impact factor: 3.365

10.  The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro.

Authors:  Dimitra Serfiotis-Mitsa; Andrew P Herbert; Gareth A Roberts; Dinesh C Soares; John H White; Garry W Blakely; Dusan Uhrín; David T F Dryden
Journal:  Nucleic Acids Res       Date:  2009-12-09       Impact factor: 16.971
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  1 in total

1.  Functional comparison of anti-restriction and anti-methylation activities of ArdA, KlcA, and KlcAHS from Klebsiella pneumoniae.

Authors:  Huimin Chen; Shuan Tao; Na Li; Fang Wang; Lei Wang; Yu Tang; Wei Liang
Journal:  Front Cell Infect Microbiol       Date:  2022-07-28       Impact factor: 6.073
  1 in total

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