Literature DB >> 31405858

Utility of Targeted, Amplicon-Based Deep Sequencing To Detect Resistance to First-Line Tuberculosis Drugs in Botswana.

Nicola M Zetola1, Sanghyuk S Shin2, Qiao Wang3, Chawangwa Modongo4,5, Christopher Allender6, David M Engelthaler6, Robin M Warren7,8,9.   

Abstract

Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid resistance among HIV-infected patients.
Copyright © 2019 American Society for Microbiology.

Entities:  

Keywords:  HIV infections; diagnostics; drug-resistant tuberculosis; heteroresistance; next-generation sequencing; single-molecule overlapping reads

Mesh：

Substances：

Year:  2019        PMID： 31405858      PMCID： PMC6811446          DOI： 10.1128/AAC.00982-19

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  14 in total

1.  Cryptic Microheteroresistance Explains Mycobacterium tuberculosis Phenotypic Resistance.

Authors:  John Z Metcalfe; Elizabeth Streicher; Grant Theron; Rebecca E Colman; Christopher Allender; Darrin Lemmer; Rob Warren; David M Engelthaler
Journal:  Am J Respir Crit Care Med       Date:  2017-11-01       Impact factor: 21.405

2.  Validation of Novel Mycobacterium tuberculosis Isoniazid Resistance Mutations Not Detectable by Common Molecular Tests.

Authors:  Justin L Kandler; Alexandra D Mercante; Tracy L Dalton; Matthew N Ezewudo; Lauren S Cowan; Scott P Burns; Beverly Metchock; Peter Cegielski; James E Posey
Journal:  Antimicrob Agents Chemother       Date:  2018-09-24       Impact factor: 5.191

3.  Detection of ultra-rare mutations by next-generation sequencing.

Authors:  Michael W Schmitt; Scott R Kennedy; Jesse J Salk; Edward J Fox; Joseph B Hiatt; Lawrence A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  2012-08-01       Impact factor: 11.205

4.  Can molecular methods detect 1% isoniazid resistance in Mycobacterium tuberculosis?

Authors:  Dorte Bek Folkvardsen; Erik Svensson; Vibeke Ø Thomsen; Erik Michael Rasmussen; Didi Bang; Jim Werngren; Sven Hoffner; Doris Hillemann; Leen Rigouts
Journal:  J Clin Microbiol       Date:  2013-02-27       Impact factor: 5.948

5.  Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis.

Authors:  A Telenti; P Imboden; F Marchesi; D Lowrie; S Cole; M J Colston; L Matter; K Schopfer; T Bodmer
Journal:  Lancet       Date:  1993-03-13       Impact factor: 79.321

6.  Genotype MTBDRplus for direct detection of Mycobacterium tuberculosis and drug resistance in strains from gold miners in South Africa.

Authors:  Susan E Dorman; Violet N Chihota; James J Lewis; Minty van der Meulen; Barun Mathema; Natalie Beylis; Katherine L Fielding; Alison D Grant; Gavin J Churchyard
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

7.  Evaluation of two line probe assays for rapid detection of Mycobacterium tuberculosis, tuberculosis (TB) drug resistance, and non-TB Mycobacteria in HIV-infected individuals with suspected TB.

Authors:  Anne F Luetkemeyer; Michelle A Kendall; Xingye Wu; Maria Cristina Lourenço; Ute Jentsch; Susan Swindells; Sarojini S Qasba; Jorge Sanchez; Diane V Havlir; Beatriz Grinsztejn; Ian M Sanne; Cynthia Firnhaber
Journal:  J Clin Microbiol       Date:  2014-01-15       Impact factor: 5.948

Review 8.  Infectiousness, reproductive fitness and evolution of drug-resistant Mycobacterium tuberculosis.

Authors:  S Borrell; S Gagneux
Journal:  Int J Tuberc Lung Dis       Date:  2009-12       Impact factor: 2.373

9.  Detection of Low-Level Mixed-Population Drug Resistance in Mycobacterium tuberculosis Using High Fidelity Amplicon Sequencing.

Authors:  Rebecca E Colman; James M Schupp; Nathan D Hicks; David E Smith; Jordan L Buchhagen; Faramarz Valafar; Valeriu Crudu; Elena Romancenco; Ecaterina Noroc; Lynn Jackson; Donald G Catanzaro; Timothy C Rodwell; Antonino Catanzaro; Paul Keim; David M Engelthaler
Journal:  PLoS One       Date:  2015-05-13       Impact factor: 3.240

10.  Protocol for a population-based molecular epidemiology study of tuberculosis transmission in a high HIV-burden setting: the Botswana Kopanyo study.

Authors:  N M Zetola; C Modongo; P K Moonan; E Click; J E Oeltmann; J Shepherd; A Finlay
Journal:  BMJ Open       Date:  2016-05-09       Impact factor: 2.692
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.