Ling Nie1, He Zhang2, Aishu Ren3, Yuzhou Li4, Gang Fu5, Richard David Cannon6, Ping Ji7, Xiaohong Wu8, Sheng Yang9. 1. College of Stomatology, Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: NieLing@stu.cqmu.edu.cn. 2. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: kqzhanghe@hospital.cqmu.edu.cn. 3. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: 500220@hospital.cqmu.edu.cn. 4. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. 5. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: 500137@hospital.cqmu.edu.cn. 6. Sir John Walsh Research Institute, University of Otago, Faculty of Dentistry, PO Box 56, Dunedin 9054, New Zealand. Electronic address: richard.cannon@otago.ac.nz. 7. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: Jiping62@hotmail.com. 8. Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: 500222@hospital.cqmu.edu.cn. 9. College of Stomatology, Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Stomatological Hospital of Chongqing Medical University, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, 426 Songshi North Road, Yubei District, Chongqing 401147, PR China. Electronic address: ysdentist@hospital.cqmu.edu.cn.
Abstract
OBJECTIVE: To fabricate a novel nano-hydroxyapatite mineralized silk fibroin (MSF) scaffold in order to diminish the resorption of alveolar ridge and accelerate new bone formation within tooth sockets. Also, to investigate the biocompatibility and osteogenic ability of the MSF in vitro, and the effect of site preservation of the MSF graft in post-extractive sockets in vivo. METHODS: SEM, EDX, FTIR and XRD were used to analyze the mineral crystals deposited on the silk fibroin (SF) surface. Pre-osteoblasts (MC3T3-E1) were seeded on SF and MSF scaffolds. Cell viability, distribution and differentiation were examined using a live-dead assay, histological analysis and Alizarin Red S staining. Furthermore, prepared grafts (SF or MSF scaffold) were implanted into the maxillary right first molar sockets of Sprague Dawley rats for 6 weeks and newly formed bone tissue was analyzed by micro-CT and histological examination. RESULTS: The SEM, EDX, FTIR and XRD analysis demonstrated that granulate nano-hydroxyapatite (nHA) crystals were uniformly distributed on the SF scaffold. In addition, the MSF hydrophilicity measured by water contact angle and swelling ratio was superior to plain SF scaffold. The effect of nHA inorganic crystals on osteogenic differentiation of MC3T3-E1 cells indicated the MSF scaffolds improved osteogenesis. Furthermore, MSF grafts induced more bone formation and reduced the height of alveolar bone resorption after tooth extraction. SIGNIFICANCE: The MSF scaffold partially simulated the structure and composition of natural bone matrix. It induced osteogenic differentiation of MC3T3-E1 cells in vitro, and also promoted new bone regeneration in tooth extraction sockets in vivo, indicating it is a biomaterial with great potential for tooth extraction site preservation.
OBJECTIVE: To fabricate a novel nano-hydroxyapatite mineralized silk fibroin (MSF) scaffold in order to diminish the resorption of alveolar ridge and accelerate new bone formation within tooth sockets. Also, to investigate the biocompatibility and osteogenic ability of the MSF in vitro, and the effect of site preservation of the MSF graft in post-extractive sockets in vivo. METHODS: SEM, EDX, FTIR and XRD were used to analyze the mineral crystals deposited on the silk fibroin (SF) surface. Pre-osteoblasts (MC3T3-E1) were seeded on SF and MSF scaffolds. Cell viability, distribution and differentiation were examined using a live-dead assay, histological analysis and Alizarin Red S staining. Furthermore, prepared grafts (SF or MSF scaffold) were implanted into the maxillary right first molar sockets of Sprague Dawley rats for 6 weeks and newly formed bone tissue was analyzed by micro-CT and histological examination. RESULTS: The SEM, EDX, FTIR and XRD analysis demonstrated that granulate nano-hydroxyapatite (nHA) crystals were uniformly distributed on the SF scaffold. In addition, the MSF hydrophilicity measured by water contact angle and swelling ratio was superior to plain SF scaffold. The effect of nHA inorganic crystals on osteogenic differentiation of MC3T3-E1 cells indicated the MSF scaffolds improved osteogenesis. Furthermore, MSF grafts induced more bone formation and reduced the height of alveolar bone resorption after tooth extraction. SIGNIFICANCE: The MSF scaffold partially simulated the structure and composition of natural bone matrix. It induced osteogenic differentiation of MC3T3-E1 cells in vitro, and also promoted new bone regeneration in tooth extraction sockets in vivo, indicating it is a biomaterial with great potential for tooth extraction site preservation.
Authors: Jun-Ting Gu; Kai Jiao; Jing Li; Jian-Fei Yan; Kai-Yan Wang; Fu Wang; Yan Liu; Franklin R Tay; Ji-Hua Chen; Li-Na Niu Journal: Bioact Mater Date: 2021-12-26