Maryam A Alghilan1, Frank Lippert2, Jeffrey A Platt3, George J Eckert4, Carlos González-Cabezas5, Daniel Fried6, Anderson T Hara7. 1. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing St., Indianapolis, IN 46202, USA; Department of Restorative Dental Science, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. Electronic address: malghila@iupui.edu. 2. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing St., Indianapolis, IN 46202, USA. Electronic address: flippert@iu.edu. 3. Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, 1121 West Michigan Street, Rm 266, Indianapolis, IN 46202, USA. Electronic address: jplatt2@iu.edu. 4. Department of Biostatistics, Indiana University School of Medicine. 410 West 10th Street, Suite 3000, Indianapolis, IN 46202, USA. Electronic address: geckert@iu.edu. 5. Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N University Ave, Ann Arbor, MI 48109, USA. Electronic address: carlosgc@umich.edu. 6. Department of Preventive and Restorative Dental Science, University of California, San Francisco School of Dentistry, 707 Parnassus Ave, San Francisco, CA 94143, USA. Electronic address: Daniel.Fried@ucsf.edu. 7. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing St., Indianapolis, IN 46202, USA. Electronic address: ahara@iu.edu.
Abstract
OBJECTIVES: Enamel thickness determination by Cross-Polarization Optical Coherence Tomography (CP-OCT) is a promising approach for quantitative monitoring of tooth wear progression. This study evaluated the ability of CP-OCT to quantify the thickness of natural enamel before, during and after tooth wear simulation. MATERIALS AND METHODS: Natural, unpolished human dental enamel slabs were submitted to five wear stages (Wear 1: to level the surfaces; Wear 2 to Wear 5: 0.05±0.02mm reduction each) simulated by an automatic grinding/polishing machine. Enamel thickness was evaluated with CP-OCT and a gold-standard method (micro-CT) at baseline and after every wear stage. Data were analyzed using ANOVA with pairwise comparisons for wear stages' impact on the thickness and wear depth measurements. The inter-method agreement was analyzed using intra-class correlation coefficients, the difference between means, and Bland-Altman plots. RESULTS: Enamel thickness measurements (mean±standard error, in mm) with natural (1.40±0.05) and worn surfaces (1.08±0.02) by CP-OCT did not differ significantly from those measured by micro-CT (natural=1.39±0.05; worn=1.09±0.02; p-values=0.30 and 0.39, respectively). CP-OCT and micro-CT showed excellent agreement on natural (ICC=0.98) and worn surfaces (ICC=0.98) enamel thickness measurements. Among and between wear stages, there were significant differences in enamel thickness and wear depth measurements for both methods (p-value <0.0001 for all). Both methods yielded similar measurements' mean (0.14±0.01; p-value=0.87) and were in good agreement (ICC=0.77) for wear depth estimation. SIGNIFICANCE: CP-OCT allows accurate measurement of enamel thickness on natural tooth surfaces. Enamel thickness measurement by CP-OCT allows quantitative monitoring of enamel thickness changes and wear depth following progressive wear.
OBJECTIVES: Enamel thickness determination by Cross-Polarization Optical Coherence Tomography (CP-OCT) is a promising approach for quantitative monitoring of tooth wear progression. This study evaluated the ability of CP-OCT to quantify the thickness of natural enamel before, during and after tooth wear simulation. MATERIALS AND METHODS: Natural, unpolished human dental enamel slabs were submitted to five wear stages (Wear 1: to level the surfaces; Wear 2 to Wear 5: 0.05±0.02mm reduction each) simulated by an automatic grinding/polishing machine. Enamel thickness was evaluated with CP-OCT and a gold-standard method (micro-CT) at baseline and after every wear stage. Data were analyzed using ANOVA with pairwise comparisons for wear stages' impact on the thickness and wear depth measurements. The inter-method agreement was analyzed using intra-class correlation coefficients, the difference between means, and Bland-Altman plots. RESULTS: Enamel thickness measurements (mean±standard error, in mm) with natural (1.40±0.05) and worn surfaces (1.08±0.02) by CP-OCT did not differ significantly from those measured by micro-CT (natural=1.39±0.05; worn=1.09±0.02; p-values=0.30 and 0.39, respectively). CP-OCT and micro-CT showed excellent agreement on natural (ICC=0.98) and worn surfaces (ICC=0.98) enamel thickness measurements. Among and between wear stages, there were significant differences in enamel thickness and wear depth measurements for both methods (p-value <0.0001 for all). Both methods yielded similar measurements' mean (0.14±0.01; p-value=0.87) and were in good agreement (ICC=0.77) for wear depth estimation. SIGNIFICANCE: CP-OCT allows accurate measurement of enamel thickness on natural tooth surfaces. Enamel thickness measurement by CP-OCT allows quantitative monitoring of enamel thickness changes and wear depth following progressive wear.
Authors: Maria Jacinta Rosario H Romero; Savio J C Bezerra; Daniel Fried; Vincent Yang; Frank Lippert; George J Eckert; Domenick T Zero; Anderson Takeo Hara Journal: J Biophotonics Date: 2021-06-17 Impact factor: 3.390