Abolfazl Abbaszadeh1, Akram Tehmasebi-Foolad2, Asghar Rajabzadeh3, Nasim Beigi-Brojeni4, Leila Zarei3. 1. Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran. 2. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran. 3. Department of Anatomical Sciences, Lorestan University of Medical Sciences, Kharamabad, Iran. 4. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Abstract
OBJECTIVE: The present study was aimed at assessment of effect of application of Chitosan/Nano Selenium biofilm on infected wound healing in rats. METHODS: Sixty-eight male Wistar rats were randomized into four groups of 17 animals each. In group I (Normal) the wounds were created with no infection. In group II (MRSA), the wounds were infected with methicillin resistant Staphylococcus aureus (MRSA). In group III (MRSA/CHIT), animals with infected wounds were dressed with chitosan biofilm only. In group IV (MRSA/CHIT/NS), animals with infected wounds were dressed with Chitosan/Nano Selenium biofilm. RESULTS: There were significant differences in comparisons of group IV and other groups, particularly in terms of cellular infiltration and neovascularization. During the study period, scores for neovascularization was significantly higher in group IV rats than other groups (P<0.05). Polymorphonuclear (PMN) and mononuclear (MNC) cell count and fibroblast cell proliferation in group IV were significantly higher than those of other experimental groups (P<0.05). CONCLUSION: Chitosan/Nano Selenium biofilm resulted in significant improvement in histopathological indices in full thickness infected wound healing.
OBJECTIVE: The present study was aimed at assessment of effect of application of Chitosan/Nano Selenium biofilm on infected wound healing in rats. METHODS: Sixty-eight male Wistar rats were randomized into four groups of 17 animals each. In group I (Normal) the wounds were created with no infection. In group II (MRSA), the wounds were infected with methicillin resistant Staphylococcus aureus (MRSA). In group III (MRSA/CHIT), animals with infected wounds were dressed with chitosan biofilm only. In group IV (MRSA/CHIT/NS), animals with infected wounds were dressed with Chitosan/Nano Selenium biofilm. RESULTS: There were significant differences in comparisons of group IV and other groups, particularly in terms of cellular infiltration and neovascularization. During the study period, scores for neovascularization was significantly higher in group IV rats than other groups (P<0.05). Polymorphonuclear (PMN) and mononuclear (MNC) cell count and fibroblast cell proliferation in group IV were significantly higher than those of other experimental groups (P<0.05). CONCLUSION: Chitosan/Nano Selenium biofilm resulted in significant improvement in histopathological indices in full thickness infected wound healing.
Entities:
Keywords:
Chitosan/Nano Selenium; Infected wound; biofilm; rat
Authors: Abul Kalam Azad; Niwet Sermsintham; Suwalee Chandrkrachang; Willem Frans Stevens Journal: J Biomed Mater Res B Appl Biomater Date: 2004-05-15 Impact factor: 3.368
Authors: Boreddy Srinivas Reddy; R Kiran Kumar Reddy; V G M Naidu; K Madhusudhana; Sachin B Agwane; Sistla Ramakrishna; Prakash V Diwan Journal: J Ethnopharmacol Date: 2007-10-13 Impact factor: 4.360
Authors: R Monina Klevens; Melissa A Morrison; Joelle Nadle; Susan Petit; Ken Gershman; Susan Ray; Lee H Harrison; Ruth Lynfield; Ghinwa Dumyati; John M Townes; Allen S Craig; Elizabeth R Zell; Gregory E Fosheim; Linda K McDougal; Roberta B Carey; Scott K Fridkin Journal: JAMA Date: 2007-10-17 Impact factor: 56.272