Literature DB >> 3139075

Genetic transformation of Streptococcus thermophilus by electroporation.

G A Somkuti1, D H Steinberg.   

Abstract

A rapid and convenient electroporation procedure was developed for the genetic transformation of intact cells of Streptococcus thermophilus with various species of plasmid DNA. Transformation frequency was influenced by the capacitance and voltage selected for electric pulsing, the pH and composition of the electroporation medium and the molecular mass of the transforming DNA. Electroporation is a simple and effective technique to introduce plasmid DNA into S. thermophilus and useful in the development of recombinant DNA technology for this important industrial microorganism.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3139075     DOI： 10.1016/0300-9084(88)90095-8

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  13 in total

1.  Molecular analysis of the glutamate decarboxylase locus in Streptococcus thermophilus ST110.

Authors:  G A Somkuti; J A Renye; D H Steinberg
Journal:  J Ind Microbiol Biotechnol       Date:  2012-03-17       Impact factor: 3.346

2.  Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation.

Authors:  D A McIntyre; S K Harlander
Journal:  Appl Environ Microbiol       Date:  1989-03       Impact factor: 4.792

3.  Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning.

Authors:  S David; G Simons; W M De Vos
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

4.  Improved electroporation efficiency of intact Lactococcus lactis subsp. lactis cells grown in defined media.

Authors:  D A McIntyre; S K Harlander
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

Review 5.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

6.  Characterization of a bacteriocin produced by Streptococcus thermophilus ST134.

Authors:  D J Ward; G A Somkuti
Journal:  Appl Microbiol Biotechnol       Date:  1995 May-Jun       Impact factor: 4.813

7.  Characterization of the Highly Autolytic Lactococcus lactis subsp. cremoris Strains CO and 2250.

Authors:  H R Riepe; C J Pillidge; P K Gopal; L L McKay
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

8.  Directed genomic integration, gene replacement, and integrative gene expression in Streptococcus thermophilus.

Authors:  B Mollet; J Knol; B Poolman; O Marciset; M Delley
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

9.  Pediocin production in milk by Pediococcus acidilactici in co-culture with Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus.

Authors:  George A Somkuti; Dennis H Steinberg
Journal:  J Ind Microbiol Biotechnol       Date:  2009-10-21       Impact factor: 3.346

10.  Isolation and characterization of chromosomal promoters of Streptococcus salivarius subsp. thermophilus.

Authors:  P Slos; J C Bourquin; Y Lemoine; A Mercenier
Journal:  Appl Environ Microbiol       Date:  1991-05       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.