Eun-Ji Ko1,2, Young Lim Oh3, Heung Yeol Kim3, Wan Kyu Eo4, Hongbae Kim5, Mee Sun Ock1, Heui-Soo Kim2, Ki Hyung Kim6,7, Hee-Jae Cha8. 1. Departments of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea. 2. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, Republic of Korea. 3. Departments of Obstetrics and Gynecology, Kosin University College of Medicine, Busan, Republic of Korea. 4. Department of Internal Medicine, Kyung Hee University, Seoul, Republic of Korea. 5. Department of Obstetrics and Gynecology, Hallym University College of Medicine, Chuncheon, Republic of Korea. 6. Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Busan, Republic of Korea. ghkim@pusan.ac.kr. 7. Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Republic of Korea. ghkim@pusan.ac.kr. 8. Departments of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea. hcha@kosin.ac.kr.
Abstract
BACKGROUND: Hypomethylation of long interspersed nuclear element-1 (LINE-1) is closely related to certain cancers and concerns with aggressive tumor behavior. Previously, we reported LINE-1 open reading frame-1 (ORF1) protein level was significantly up-regulated in ovarian cancers compared with normal ovary. Hypomethylation of local LINE-1 sequence has been reported to reactivate MET proto-oncogene in colon cancers and hepatocellular carcinoma. However, the relationship between LINE-1 and c-MET expressions in ovarian cancer is not yet studied. METHOD: Here, we analyzed the expression patterns of LINE-1 ORF1 and c-Met protein in ovarian cancer tissue microarrays containing 208 surgical specimens including normal ovary and malignant ovarian cancers. RESULTS: The expressions of both LINE-1 ORF1 and c-Met protein were significantly increased in ovarian cancers and peaked in early stage of tumor. Other clinical data including age and tumor types were not significantly related with both proteins. Co-relationship between LINE-1 ORF1 and c-Met protein was significant (p = 0.03) but several patients show different expression patterns. CONCLUSIONS: These results propose that LINE-1 ORF1 significantly activates c-Met but not in all cases, suggesting other factors may be involved simultaneously.
BACKGROUND: Hypomethylation of long interspersed nuclear element-1 (LINE-1) is closely related to certain cancers and concerns with aggressive tumor behavior. Previously, we reported LINE-1 open reading frame-1 (ORF1) protein level was significantly up-regulated in ovarian cancers compared with normal ovary. Hypomethylation of local LINE-1 sequence has been reported to reactivate MET proto-oncogene in colon cancers and hepatocellular carcinoma. However, the relationship between LINE-1 and c-MET expressions in ovarian cancer is not yet studied. METHOD: Here, we analyzed the expression patterns of LINE-1 ORF1 and c-Met protein in ovarian cancer tissue microarrays containing 208 surgical specimens including normal ovary and malignant ovarian cancers. RESULTS: The expressions of both LINE-1 ORF1 and c-Met protein were significantly increased in ovarian cancers and peaked in early stage of tumor. Other clinical data including age and tumor types were not significantly related with both proteins. Co-relationship between LINE-1 ORF1 and c-Met protein was significant (p = 0.03) but several patients show different expression patterns. CONCLUSIONS: These results propose that LINE-1 ORF1 significantly activates c-Met but not in all cases, suggesting other factors may be involved simultaneously.
Authors: E C Koon; P C Ma; R Salgia; W R Welch; J G Christensen; R S Berkowitz; S C Mok Journal: Int J Gynecol Cancer Date: 2007-11-16 Impact factor: 3.437
Authors: J Pattamadilok; N Huapai; P Rattanatanyong; A Vasurattana; S Triratanachat; D Tresukosol; A Mutirangura Journal: Int J Gynecol Cancer Date: 2007-10-18 Impact factor: 3.437