An assessment of the ocular tear film in patients with thyroid disorders.

Objective: To investigate the ocular tear film in patients with thyroid disorders using various tests.
Methods: The study involved the assessment of the ocular tear film in 20 patients with thyroid disorders (6 men and 14 women) aged 18-43 years (mean±standard deviation=34.3±3.2 years). An age-matched control group consisting of 20 subjects (8 males and 12 females) ranging in age from 18 to 43 years (31.3±2.9 years) was also examined under similar conditions. All patients completed the Ocular Surface Disease Index questionnaire, followed by the tear ferning test within the right eye. A phenol red thread test was carried out 10 mins later followed by the fluorescein tear break-up test with a 10-min gap between the tests being implemented.
Results: The median score for the Ocular Surface Disease Index (P<0.05) showed the condition of mild dry eyes [median (IQR)=15.5 (21.9)] in patients with thyroid disorders compared to the control group [5.6 (3.6)]. The mean score for the phenol red thread test within both the right and left eyes showed acceptable tear volumes of 11.7±8.1 and 10.5±7.4 mm, respectively, but this was much lower (P<0.05) compared to those recorded within the control group (22.2±6.5 and 20.7±5.2 mm, respectively). In addition, the mean for the tear break-up time (P<0.05) scores in both eyes within the patients with thyroid disorders revealed a certain degree of eye dryness (4.9±1.6 and 4.2±1.9 s), while the control group showed normal eye scores (13.2±2.6 and 12.3±2.2 s). The median score for tear ferning grades showed eye dryness [2.0 (2.2)] within the study group and normal eyes [1.2 (0.9)] within the control group. Conclusions: Patients with thyroid disorders have a significant level of eye dryness compared to normal eye subjects.

Introduction

Dry eye syndrome is a very common disease of the ocular surface that is caused by various factors. It leads to tear instability due to excessive tear evaporation or a deficiency in tear secretion. The symptoms of eye dryness include eye irritation, excess tearing, discomfort, photophobia, blurred vision, a burning sensation, and redness of the eyes.1,2 The tear cycle begins with the tear secretion by the lacrimal gland followed by blinking that distributes the tears over the ocular surface. Finally, tear drainage occurs through the lacrimal puncta or via evaporation.3,4 Two main types of dry eye are known, which are, namely, aqueous tear-deficient dry eye and evaporative dry eye.2 Aqueous tear-deficient dry eye is caused due to lack of lacrimal secretion and the conjunctiva failing to secrete water, while evaporative dry eye is caused due to certain internal and external causes that serve to accelerate the evaporation of tears.2 Disorders in the meibomian gland are the most common internal cause of evaporative dry eye.5 Moreover, ocular surface disorders and the wearing of contact lenses are the most common external causes for evaporative dry eye.6 Instability in the tear film can lead to dryness since tear film stability depends on several factors, such as tear osmolarity, tear clearance, appropriate blinking, normal lacrimal function, and the maintenance of a normal ocular surface.1

The side-by-side boxplot for the TUBT (OD) within the study and control groups. Statistically significant at P<0.05.

Abbreviation: TBUT, tear break-up time.

There are various tests that can be used to diagnose dry eye along with dry eye questionnaires. Some diagnostic tests are able to detect the quantity of the tears, while others detect the quality of the tears. A combination of tests should be used since there is no single test which can be used for diagnosing the condition of dry eye.7 The most common dry eye questionnaire is the Ocular Surface Disease Index (OSDI) [1]. The most common tests used to detect the quantity of tears are the Schirmer test and the phenol red thread (PRT) tests.8,9 Incidentally, the tear ferning (TF), tear break-up time (TBUT), tear meniscus height, and osmolarity tests are the most commonly used tests that are employed in order to detect the quality of tears.10–14 The treatment of dry eye is very important if the symptoms of eye dryness are to be alleviated in patients suffering from the condition.7 Various artificial tears can be used in order to lubricate the eye surface and improve the life quality of patients.7 In addition, tears can be preserved by bringing about a reduction in their drainage and via the process of evaporation. The reduction of tears via evaporation can be achieved by increasing humidity in the environment and through avoiding direct air flow as well as the use of oily lubricants.7 Moreover, the use of supplementary nutrients such as evening primrose oil and various fish oils may also be beneficial.15

The most common types of thyroid disease are hypothyroidism and hyperthyroidism.16,17 The corneal sensitivity was reduced during the early signs of thyroid gland disorders.18 In addition, confocal microscopy indicated that the nerve fibers in the cornea were low in patients with thyroid disorders compared with normal eye subjects.19 Thyroid-associated orbitopathy (TAO), thyroid eye disease, and Graves’ ophthalmopathy (GO) are examples of orbital inflammatory conditions that are often associated with dry eye syndrome. Significantly, TAO patients suffer from eye discomfort, redness, irritation, foreign body sensation, and fibrosis in the tissue of the orbit. Annually, TAO occurs in approximately 16 women and 3 men in every 100,000. TAO is common in patients suffering from Graves’ disease (80%), but it is not unusual in hypothyroid patients.20 Studies have shown that 45–85% of the TAO patients have dry eye syndrome.20 The widening of the palpebral fissure, exophthalmos, tear production deficiency, and tear evaporation are the main contributing factors for this eye dryness.21–24 Several reports have indicated a relationship between thyroid gland disorder and dryness of the eye.25–29 In the current study, we report on how the ocular tear film may be assessed in thyroid gland patients in Saudi Arabia as part of our containing research into the assessment of the ocular tear film.30–35

Methods

Subjects

Twenty patients with thyroid disorders (6 men and 14 women), aged 18–43 years (mean ± standard deviation=34.3±3.2 years) from Riyadh City participated in the study. The patients represented two types of thyroid disorders as hypothyroidism (N=12) and hyperthyroidism (N=8). None of the patients were diagnosed with an autoimmune thyroid disease such as Hashimoto’s thyroiditis and Graves’ disease or thyroid cancer. Moreover, an age-matched control group consisting of 20 subjects (8 males and 12 females) ranging in age from 18 to 43 years (31.3±2.9 years) was recruited and examined under similar conditions. Any patients with a history of ocular surgery or medications, those demonstrating the recent use of contact lenses, pregnant or breastfeeding women, as well as diabetics and smokers were all excluded from the study. Subjects older than 43 years were excluded since aging (43–86 years) is a known risk factor for eye dryness.36 All participants provided written informed consent, and this study was conducted in accordance with the Declaration of Helsinki. Ethical approval (ethics number CAMS-046–3839) for this study was obtained from the College of Applied Medical Science Ethics Committee, King Saud University and King Fahad Medical City. The study itself was conducted at King Fahad Medical City and King Khaled University Hospital, Riyadh. All measurements were carried out by the same examiner under normal conditions. All patients completed the OSDI sheet first, followed by the TF test in the right eye. After 10 mins, the PRT test was performed followed by the TUBT test, which was carried out in both eyes, with a 10-min gap in between. The measurements were carried out between the hours of 7:30 am to 3:30 pm. No significant difference in tear quality between measurements was recorded even though the diagnostic tests were carried out at different periods during a single testing session.32

Thyroid gland disorder diagnosis

The diagnosis of thyroid gland disorders was based on the serum thyroid-stimulating hormone (TSH) and free thyroxine (FT4) levels. High levels of TSH and low levels of FT4 indicate hypothyroidism. On the other hand, low levels of TSH and high levels of FT4 indicate hyperthyroidism.37 The manufacturer’s reference range for TSH is 0.27–4.20 µIU/mL. However, the range for FT4 is 12–22 pmol/L (0.93–1.7 ng/dL).

OSDI sheet

An OSDI score of 12 was considered to be an indication of a normal healthy eye, a score of 13–22 was considered to be an indication of a mild dry eye condition, 23–32 was considered to be a sign of moderate dye eye condition, and a score of more than 33 indicated severe eye dryness.38

TF test

A glass capillary tube (10 µL, Sigma-Aldrich Chemical Company, UK) was used to collect a tear sample (1 µL) from the lower meniscus of the right eye from each subject. The tears were expelled onto a glass slide to dry for 10 mins under normal environmental conditions (23°C and a humidity level of less than 40%). The TF patterns produced were observed under a digital microscope (Olympus DP72, Tokyo, Japan) with 10×magnifications. The five-point tear ferning grading scale was used to grade the tear ferning patterns using the 0.1 increments in which the cut-off for dry eye is ≥2.10

PRT test

PRT strips (Zone-Quick) were purchased from Showa Yakuhin Kako Co, Ltd (Tokyo, Japan). The thread changes color (from a yellow to a light red) when moistened by tears as a result of the change in pH due to tears. A portion (approximately 3-mm in length) of the thread was folded and inserted one-third of the distance from the temporal canthus of the lower eyelid, with the eye in the primary position. After 15 s, the thread was gently removed and the red-colored portion was measured in millimmeters. The procedure was performed first on the left eye followed by the right eye with a 5-min gap between measurements.30 Dry eye was defined for wetting readings less than 10 mm.

TBUT test

The TBUT test was performed using ProGlo® Fluorescein Strips (Eye Care & Cure, Tucson, USA). The subject was asked to look down, and a strip was gently touched against the superior conjunctiva. The subject was asked to blink several times and to keep the eye open whilst fixing his/her gaze on a distant target. The time interval between the last blink and the first appearance of dark spots on the pre-corneal tear film was recorded in seconds. The measurements were taken in both eyes. The cut-off value for dry eye is <10 s.39

Statistical analysis

The data were tabulated using Excel (Microsoft™ Office 2010, Microsoft Corp., Redmond, USA) and was analyzed using the SPSS® statistical package, version 22.0 (SPSS Inc., Chicago, IL, USA) for Windows®. A correlation coefficient was used to investigate the relationship between the obtained data.40

Results

Twenty patients with thyroid disorders participated as the study group, and an age-matched control group of 20 patients completed the study. The data were found to be normally distributed (Kolmogorov–Smirnov test; P>0.05) for the PRT and TBUT scores, and therefore, the mean±standard deviation (SD) results were used to represent the average. The data were found to be abnormally distributed (Kolmogorov–Smirnov test; P˂0.05) for the OSDI and TF scores and, therefore, the median (interquartile range) were used to represent the average. The mean±SD values for the PRT and TBUT scores and the median (IQR) for the OSDI and TF scores are shown in Table 1.

Table 1

Average [mean±standard deviation or median (interquartile range)] for the OSDI, PRT1, PRT2, TBUT1, TBUT2, and TF scores for the study and control groups

Test	Average
Study group (N=20)*	Control group (N=20)
OSDI score	15.5 (21.9)	5.6 (3.6)
PRT1 (mm)	11.7±8.1	22.2±6.5
PRT2 (mm)	10.5±7.4	20.7±5.2
TBUT1 (s)	4.9±1.6	13.2±2.6
TBUT2 (s)	4.2±1.9	12.3±2.2
TF grade	2.0 (2.2)	1.2 (0.9)

Note: *Statistically significant value at P<0.05.

Abbreviations: OSDI, Ocular Surface Disease Index; PRT, phenol red thread; TBUT, tear break-up time; TF, tear ferning; PC, Pearson correlation coefficient; Sig., significance (two-tailed).

The median score for the OSDI (P<0.05) in the study group showed a mild dry eye condition [15.5 (21.9)], while the average score within the control group showed a normal eye condition [5.6 (3.6)]. For the study group, the mean score for the PRT tests within both the right and left eyes showed an acceptable tear volume (11.7±8.1 and 10.5±7.4 mm, respectively), but this was significantly lower (P<0.05) than the volumes found within the control group (22.2±6.5 and 20.7±5.2 mm, respectively). In addition, the mean for the TBUT scores within the study group showed eye dryness (4.9±1.6 and 4.2±1.9 mm for the right and left eyes, respectively). Normal eyes were indicated from the TBUT scores within the control group (13.2±2.6 and 12.3±2.2 s for the right and left eyes, respectively). The average score for the TF grades (P<0.05) showed eye dryness [2.0 (2.2)] within the study group and showed normal eye conditions [1.2 (0.9)] within the control group. The side-by-side boxplots for the PRT, TBUT and TF scores within both the study and control groups are shown in Figures 1–3, respectively. Examples of TF images obtained from both the study and the control groups are shown in Figures 4 and 5, respectively.

Figure 1

The side-by-side boxplot for the PRT (OD) within the study and control groups. Statistically significant at P<0.05.

Abbreviation: PRT, phenol red thread.

Figure 3

The side-by-side boxplot for the TF test within the study and control groups. Statistically significant at P<0.05.

Abbreviation: TF, tear ferning.

Figure 4

Examples of TF images obtained from the study group (showing dry eye conditions in most cases).

Abbreviation: TF, tear ferning.

Figure 5

Examples of TF images obtained from the control group (showing normal eye conditions in all cases).

Abbreviation: TF, tear ferning.

The correlations between the scores from the various tests carried out within the study group are recorded in Table 2. Generally, the correlations (Mann–Whitney test) between tests were weak since each test corresponds to a different parameter. The OSDI has a negative weak correlation (r=–0.111) with the PRT scores, a positive weak correlation (r=0.085) with the TF grades, and a medium negative correlation (r=–0.373) with TBUT scores. The PRT scores have a positive medium correlation with the TBUT scores (r=0.371) and the TF grades (r=0.023).

Table 2

Correlation of the OSDI, PRT, TBUT (OD), and TF scores for the study group (N=20)*

Test/correlation		OSDI	PRT	TBUT	TF
OSDI	PC	1	–0.111	–0.373	0.085
	Sig	—	0.661	0.127	0.738
PRT	PC	–0.111	1	0.371	0.023
	Sig	0.661	—	0.130	0.927
TBUT	PC	–0.373	0.371	1	0.033
	Sig	0.127	0.130	—	0.898
TF	PC	0.085	0.023	0.033	1
	Sig	0.738	0.927	0.898	—

Note: *Statistically significant value at P<0.05.

Abbreviations: OSDI, Ocular Surface Disease Index; PRT, phenol red thread; TBUT, tear break-up time; TF, tear ferning; PC, Pearson correlation coefficient; Sig., significance (two-tailed).

The patients with thyroid gland disorders (N=20) were classified as hypothyroidism (N=12) and hyperthyroidism (N=8). The mean±SD scores for the PRT and TBUT tests and the median (IQR) scores for the OSDI and TF for both hypothyroidism and hyperthyroidism subjects are shown in Table 3. The TBUT and TF scores showed no significant (P>0.05) differences between hypothyroidism and hyperthyroidism subjects. The OSDI score was significantly (P<0.05) lower in hypothyroidism subjects, while the PRT scores were significantly (P<0.05) lower in hyperthyroidism subjects.

Table 3

Average (mean±standard deviation or median [interquartile range]) for the OSDI, PRT1, PRT2, TBUT1, TBUT2, and TF scores within hypothyroidism and hyperthyroidism subjects

Test	Average
Hypothyroidism (N=8)	Hyperthyroidism (N=12)
OSDI score*	14.3 (10.0)	25.8 (30.1)
PRT1 (mm)*	13.5±9.4	9.4±5.8
PRT2 (mm)*	12.5±7.0	8.0±6.6
TBUT1 (s)	4.3±1.4	5.4±1.5
TBUT2 (s)	3.9±1.7	4.5±1.8
TF grade	2.0 (15)	2.5 (3.0)

Note: *Statistically significant value at P<0.05.

Abbreviations: PRT, phenol red thread; TBUT, tear break-up time; TF, tear ferning; PRT1 and TBUT1, the measurements for the right eye; PRT2, the measurements for the left eye; TBUT2, the tears for the TF test were collected from the right eye.

Discussion

The results obtained revealed that patients with thyroid disorders had a significant dry eye level compared to the control subjects. The OSDI score for patients with thyroid disorders was high compared with the score for the control group. The mean score for the PRT test within both the right and left eye for the study group was much lower (11.7±8.1 and 10.5±7.4 mm, respectively) when compared with the scores observed within the control group (22.2±6.5 and 20.7±5.2 mm, respectively). Similarly, the mean score for the TBUT test for the study group in both eyes was significantly lower (4.9±1.6 and 4.2±1.9 s) compared with those obtained within the control group (13.2±2.6 and 12.3±2.2 s). The main score for the TF grades showed a level of eye dryness [2.0 (2.2)] for the study group, while the main score for the control group showed normal eye conditions [1.2 (0.9)]. To the best of our knowledge, this is the first time that a TF test was used to assess the quality of tears within patients with thyroid disorders in Saudi Arabia.

The current study showed that the median average for the OSDI score was higher in hyperthyroidism subjects (25.8 [30.1]) compared with hypothyroidism subjects (14.3 [10.0]). While, the main scores for the PRT test within both eyes were higher in hypothyroidism subjects compared with those hyperthyroidism subjects. Similar observations were made before within hypothyroidism and hyperthyroidism subjects.21 For example, the average OSDI score was found to be significantly higher in hyperthyroidism subjects [31.0 (28.0)] compared with hypothyroidism subjects [11.2 (11.0)].21 While, tear evaporation rate was found to significantly higher in hypothyroidism subjects [53.0 (28.0)] compared with hyperthyroidism subjects [29.0 (59.5)].21 The high level of dryness within patients with thyroid disorders could possibly be due to inadequate tear production, high tear evaporation, tear film instability, and disturbances within the content of certain proteins within the tear film.

The evaluation and management of GO and TAO patients have been discussed previously.41,42 Patients with thyroid disorders tend to suffer from eye dryness as well as significant damage to the ocular surface compared with normal patients. The OSDI scores in TAO patients are noted to be high in comparison with the control group.22,24 For example, the mean OSDI score recorded in relation to patients with thyroid disorders was higher (29.0±20.8) compared with the one recorded for normal eye subjects (13.2±7.8).22 The mean score for the Schirmer test within the patients with thyroid disorders was 5.1±2.3 mm compared to 11.4±3.3 mm for the control group.22 Another study showed that the mean score for the Schirmer test was 14. ±8.3 mm in patients with thyroid disorders, which is significantly lower than the one recorded within normal eye subjects (24.9±3.6 mm).25 The mean TBUT scores in the patients with thyroid disorders were found to be 6.8±3.7 s compared with 12.7±1.9 s for the control group subjects.22 The mean average for the TBUT test was found to be lower within patients with thyroid disorders (5.8–7.1 s) compared with the score recorded within the control group (10.0–11.4 s).22,25–27 Moreover, the tear osmolarity was found to be higher in patients with thyroid disorders (319.0±17.4 mOsm/L) compared to the control group (307.9±12.7 mOsm/L).22

Patients with thyroid disorders suffer from exophthalmos that affects the soft tissues of the eye leading to their expansion.43,44 Exophthalmos leads to lagophthalmos; a condition which renders an individual unable to fully close their eyelids. This subsequently disturbs the ocular surface and elevates the tear evaporation rate as well as tear hyperosmolarity.45,46 In addition, strabismus is common in patients with thyroid disorders as a result of the impairment of the extraocular muscles, which results in head tilt and diplopia. Diplopia usually occurs in patients with thyroid disorders due to the inflammation and swelling of the extraocular muscles.47 The most affected extraocular muscles are the inferior rectus and the medial rectus muscles, resulting in vertical and horizontal deviations. Therefore, due to the changes in tear evaporation rate, tear osmolarity, and altered eyelid structure, dry eye syndrome is usually seen in patients with thyroid disorders.47 The onset of the various different signs and symptoms of patients with thyroid disorders differs amongst patients. They can be seen simultaneously or take up to several months or years to manifest themselves.48

Evidence has emerged to suggest that the ocular inflammation mediated by T lymphocytes plays a critical role in the pathogenesis of dry eye syndrome by reducing the production of aqueous tears.49,50 Patients with thyroid disorders have an abnormality in the proteinaceous composition and protein profile of the tear film, which indicates a dysfunction in the lacrimal gland as highlighted by certain studies.51–53 The damage sustained by the ocular surface in patients with thyroid disorders is associated with a reduction in tear production due to the involvement of the lacrimal gland.27 In addition, the instability of the tear film results in a shorter tear film breakup time, which is related to palpebral fissure height in patients with thyroid disorders.54

Conclusion

Patients with thyroid disorders were found to have significantly high levels of eye dryness due to factors associated with dry eye syndrome. The mechanism by which such eye dryness occurs is not yet clear, although several factors have been cited including low tear production, excessive tear evaporation, tear instability, and disturbances in the tear film lipid layer. A more detailed study is needed in order to better understand the situation and which factors cause such phenomena to occur. Eye drops and lubricants may possibly help patients with thyroid disorders by reducing their ocular discomfort and by smoothing the ocular surface, thereby providing better vision and relief from such debilitating symptoms.