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Literature DB >> 31387789

A glycal-based photoaffinity probe that enriches sialic acid binding proteins.

Abstract

To identify sialic acid binding proteins from complex proteomes, three photocrosslinking affinity-based probes were constructed using Neu5Ac (5 and 6) and Neu5Ac2en (7) scaffolds. Kinetic inhibition assays and Western blotting revealed the Neu5Ac2en-based 7 to be an effective probe for the labeling of a purified gut microbial sialidase (BDI_2946) and a purified human sialic acid binding protein (hCD33). Additionally, LC-MS/MS affinity-based protein profiling verified the ability of 7 to enrich a low-abundance sialic acid binding protein (complement factor H) from human serum thus validating the utility of this probe in a complex context.

Entities: Chemical Disease Gene Species

Keywords: Activity-based probe; Lectin; Neu5Ac; Sialic acid; Sialidase

Mesh：

Substances：

Year: 2019 PMID： 31387789 PMCID： PMC6730559 DOI： 10.1016/j.bmcl.2019.07.054

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

52 in total

1. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry.

Authors: Joshua N Adkins; Susan M Varnum; Kenneth J Auberry; Ronald J Moore; Nicolas H Angell; Richard D Smith; David L Springer; Joel G Pounds
Journal: Mol Cell Proteomics Date: 2002-12 Impact factor: 5.911

2. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host-microbial interactions.

Authors: Malin E V Johansson; Jessica M Holmén Larsson; Gunnar C Hansson
Journal: Proc Natl Acad Sci U S A Date: 2010-06-25 Impact factor: 11.205

3. Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae.

Authors: Boris Zybailov; Amber L Mosley; Mihaela E Sardiu; Michael K Coleman; Laurence Florens; Michael P Washburn
Journal: J Proteome Res Date: 2006-09 Impact factor: 4.466

Review 4. Gastrointestinal microbiology enters the metagenomics era.

Authors: Daniel N Frank; Norman R Pace
Journal: Curr Opin Gastroenterol Date: 2008-01 Impact factor: 3.287

5. Photoactivatable crosslinking sugars for capturing glycoprotein interactions.

Authors: Yoshihito Tanaka; Jennifer J Kohler
Journal: J Am Chem Soc Date: 2008-02-23 Impact factor: 15.419

Review 6. Inflammatory bowel disease: clinical aspects and established and evolving therapies.

Authors: Daniel C Baumgart; William J Sandborn
Journal: Lancet Date: 2007-05-12 Impact factor: 79.321

7. Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling.

Authors: Zhengqiu Li; Piliang Hao; Lin Li; Chelsea Y J Tan; Xiamin Cheng; Grace Y J Chen; Siu Kwan Sze; Han-Ming Shen; Shao Q Yao
Journal: Angew Chem Int Ed Engl Date: 2013-06-10 Impact factor: 15.336

A glycal-based photoaffinity probe that enriches sialic acid binding proteins.

1. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry.

2. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host-microbial interactions.

3. Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae.

Review 4. Gastrointestinal microbiology enters the metagenomics era.

5. Photoactivatable crosslinking sugars for capturing glycoprotein interactions.

Review 6. Inflammatory bowel disease: clinical aspects and established and evolving therapies.

7. Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling.

Review 8. Activity-based protein profiling: from enzyme chemistry to proteomic chemistry.

Review 9. Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions.

10. Cell-permeable probe for identification and imaging of sialidases.

1. Influence of protecting groups on O- and C-glycosylation with neuraminyl and ulosonyl dibutylphosphates.

2. A photoaffinity probe that targets folate-binding proteins.

3. Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them.