| Literature DB >> 31384044 |
Zilu Ye1, Yang Mao1,2, Henrik Clausen1, Sergey Y Vakhrushev3.
Abstract
We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins. This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification). The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures. Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.Entities:
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Year: 2019 PMID： 31384044 DOI： 10.1038/s41592-019-0504-x
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547