Isotope fractionation approach to characterize the reactive transport processes governing the fate of hexachlorocyclohexanes at a contaminated site in India.

The transformation processes of hexachlorocyclohexane isomers (HCHs) from production sites of Lindane across the landscape and along the food web were studied as an example to understand the fate of POPs in the environment. Therefore, we studied the concentration and isotope composition of HCHs in different matrices in the vicinity of a dumpsite and a chemical plant producing HCHs in India. Carbon isotope compositions (δ13C) of HCHs and the enantiomer fraction (EF) of α-HCH were used as indicators to characterize in situ degradation in soil, groundwater, and sediment as well as along the food web. The HCHs were detected in plants growing on contaminated soil. Elevated concentrations of HCHs were found in a number of crops, which indicates an important transfer pathway of HCHs entering food webs. The EF value of α-HCH and the δ13C signature of HCHs indicated that degradation processes occurred in the rhizosphere or within the plants potentially attenuating the contamination of HCHs. The isotope enrichment of HCHs in dung and milk samples showed that degradation of HCHs may take place in the digestive track of cow and buffalo as well as during their metabolism. The δ13C of HCHs was used to analyze the potential dispersion routes on the landscape scale in order to understand the reactive transport pathways starting at the source of HCHs. In this study, the potential of carbon isotope fractionation and EF for characterizing uptake of HCHs into plants and accumulation in the food web were examined. To the best of our knowledge, this is the first study using the combination of stable isotope fractionation and EF to track the reactive transport processes in a complex environment including the food web.