Literature DB >> 31378710

Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

Daniela Boassa1, Sakina P Lemieux2, Varda Lev-Ram2, Junru Hu3, Qing Xiong2, Sebastien Phan3, Mason Mackey3, Ranjan Ramachandra3, Ryan Emily Peace4, Stephen R Adams2, Mark H Ellisman5, John T Ngo6.   

Abstract

A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as "multicolor" electron microscopy (EM) via the photooxidation of 3-3'-diaminobenzidine and its derivatives.
Copyright © 2019 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  LOV domain; electron microscopy; protein-protein interactions; split-fluorescent proteins

Year:  2019        PMID： 31378710      PMCID： PMC6800643          DOI： 10.1016/j.chembiol.2019.07.007

Source DB:  PubMed          Journal:  Cell Chem Biol        ISSN： 2451-9448            Impact factor:   8.116


  29 in total

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  9 in total

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