Elizabeth S Miller1, Camille G Apple2, Kolenkode B Kannan3, Zackary M Funk4, Jessica M Plazas5, Philip A Efron6, Alicia M Mohr7. 1. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, United States. Electronic address: Elizabeth.Miller@surgery.ufl.edu. 2. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, United States. Electronic address: Camille.apple@surgery.ufl.edu. 3. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, United States. Electronic address: Kolenkode.Kannan@surgery.ufl.edu. 4. University of Florida, College of Medicine, Gainesville, FL, United States. Electronic address: zfunk123@ufl.edu. 5. University of Florida, College of Liberal Arts and Sciences, Gainesville, FL, United States. Electronic address: jplaz011@fiu.edu. 6. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, United States. Electronic address: Philip.Efron@surgery.ufl.edu. 7. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, United States. Electronic address: alicia.mohr@surgery.ufl.edu.
Abstract
INTRODUCTION: This study sought to determine if the systemic cytokine profile of rodents subjected to chronic restraint stress leads to persistent low-grade inflammation. METHODS: Male Sprague-Dawley rats were subjected to restraint stress for a total of seven or fourteen days. Urine norepinephrine (NE), plasma interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP) were assessed with ELISA. Liver expression of IL-6 and TNF-α were assessed with real time PCR. RESULTS: Chronic stress at 7 and 14 days sequentially increased plasma acute phase reactants (NE, IL-6, TNF-α, and CRP), liver IL-6 expression, hematopoietic progenitor cell mobilization, and decreased erythroid progenitor colony growth. Weight gain was reduced by chronic stress compared to each models' naïve counterpart. CONCLUSIONS: Combining this model with trauma and sepsis models will allow evaluation of the contribution of persistent inflammation in disease progression and outcomes.
INTRODUCTION: This study sought to determine if the systemic cytokine profile of rodents subjected to chronic restraint stress leads to persistent low-grade inflammation. METHODS: Male Sprague-Dawley rats were subjected to restraint stress for a total of seven or fourteen days. Urine norepinephrine (NE), plasma interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), C-reactive protein (CRP) were assessed with ELISA. Liver expression of IL-6 and TNF-α were assessed with real time PCR. RESULTS: Chronic stress at 7 and 14 days sequentially increased plasma acute phase reactants (NE, IL-6, TNF-α, and CRP), liver IL-6 expression, hematopoietic progenitor cell mobilization, and decreased erythroid progenitor colony growth. Weight gain was reduced by chronic stress compared to each models' naïve counterpart. CONCLUSIONS: Combining this model with trauma and sepsis models will allow evaluation of the contribution of persistent inflammation in disease progression and outcomes.
Authors: Camille G Apple; Elizabeth S Miller; Kolenkode B Kannan; Chase Thompson; Dijoia B Darden; Philip A Efron; Alicia M Mohr Journal: J Surg Res Date: 2021-06-26 Impact factor: 2.192
Authors: Julia Hinterdobler; Heribert Schunkert; Thorsten Kessler; Hendrik B Sager Journal: Antioxid Redox Signal Date: 2021-09-14 Impact factor: 8.401