Duo Zhou1, Meng-Ying Zhu1, Yi-Long Wang2, Xiao-Qiang Hao1, Dong-Ming Zhou3, Rong-Xian Liu1, Chu-Di Zhang1, Chu-Fan Qu1, Zheng-Yan Zhao4,5. 1. Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. 2. Department of Neurology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China. 3. Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China. 4. Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. zhaozy@zju.edu.cn. 5. Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China. zhaozy@zju.edu.cn.
Abstract
BACKGROUND: To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes. METHODS: Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity. RESULTS: Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages. CONCLUSION: rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.
BACKGROUND: To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes. METHODS: Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity. RESULTS: Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages. CONCLUSION: rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.
Entities:
Keywords:
Foreign genes; Mumps virus; Reverse genetic system
Authors: Pei Xu; Priya Luthra; Zhuo Li; Sandra Fuentes; James Alexander D'Andrea; Jianguo Wu; Steven Rubin; Paul A Rota; Biao He Journal: J Virol Date: 2011-11-16 Impact factor: 5.103