Literature DB >> 3137248

Effect of egg yolk on growth of Mycobacterium tuberculosis in 7H12 liquid medium.

Y Kononov1, K D Ta, L Heifets.   

Abstract

Of 92 drug-resistant Mycobacterium tuberculosis strains isolated from sputum specimens, 86 showed growth in two types of 7H12 broth, one with egg yolk and the other without egg yolk. In addition, two strains grew only in plain 7H12 broth without yolk, and four others were recovered only in the medium supplemented with egg yolk. The radiometrically detected growth was higher in the presence of egg yolk, corresponding to a higher number of CFU per milliliter in these cultures. The improvement of growth in 7H12 broth supplemented with egg yolk was most noticeable in cultures isolated from sputum specimens having a low number of acid-fast bacilli in the smear and producing only a few colonies on solid media.

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Year:  1988        PMID： 3137248      PMCID： PMC266617          DOI： 10.1128/jcm.26.7.1395-1397.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  A selective oleic acid albumin agar medium for tubercle bacilli.

Authors:  D A Mitchison; B W Allen; L Carrol; J M Dickinson; V R Aber
Journal:  J Med Microbiol       Date:  1972-05       Impact factor: 2.472

2.  Isolation of mycobacteria from clinical specimens by use of selective 7H11 medium.

Authors:  J K McClatchy; R F Waggoner; W Kanes; M S Cernich; T L Bolton
Journal:  Am J Clin Pathol       Date:  1976-03       Impact factor: 2.493

3.  Ethambutol MICs and MBCs for Mycobacterium avium complex and Mycobacterium tuberculosis.

Authors:  L B Heifets; M D Iseman; P J Lindholm-Levy
Journal:  Antimicrob Agents Chemother       Date:  1986-12       Impact factor: 5.191

4.  Determination of in vitro susceptibility of Mycobacterium tuberculosis to cephalosporins by radiometric and conventional methods.

Authors:  L B Heifets; M D Iseman; J L Cook; P J Lindholm-Levy; I Drupa
Journal:  Antimicrob Agents Chemother       Date:  1985-01       Impact factor: 5.191

5.  The effect of polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium.

Authors:  R R Cutler; P Wilson; F V Clarke
Journal:  Tubercle       Date:  1987-09

6.  Radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis in egg-yolk-enriched BACTEC 12A medium.

Authors:  C L Woodley; R W Smithwick
Journal:  Antimicrob Agents Chemother       Date:  1988-01       Impact factor: 5.191
  6 in total
  3 in total

1.  Effects of decontamination methods and culture conditions on viability of Mycobacterium ulcerans in the BACTEC system.

Authors:  J C Palomino; F Portaels
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

2.  Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens.

Authors:  M T Collins; K B Kenefick; D C Sockett; R S Lambrecht; J McDonald; J B Jorgensen
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

3.  Processing respiratory specimens with C18-carboxypropylbetaine: development of a sediment resuspension buffer that contains lytic enzymes to reduce the contamination rate and lecithin to alleviate toxicity.

Authors:  C G Thornton; K M MacLellan; T L Brink; D M Wolfe; O J Llorin; S Passen
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948
  3 in total

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