Di Song1, Xian-Liang Huang2, Ling Hong3, Jian-Min Yu3, Zhao-Feng Zhang4, Hui-Qin Zhang5, Zhao-Gui Sun4, Jing Du6. 1. NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai, People's Republic of China; Second Military Medical University, Changhai Hospital, Shanghai, People's Republic of China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai, People's Republic of China; Shanghai Institute of Planned Parenthood Research Hospital, Changhai Hospital, Shanghai, People's Republic of China. 3. Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China. 4. NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai, People's Republic of China. 5. Second Military Medical University, Changhai Hospital, Shanghai, People's Republic of China. 6. NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai, People's Republic of China. Electronic address: dujing42@126.com.
Abstract
OBJECTIVE: To examine whether sequence variants within the FSHR and CYP19A1 genes are related to the ovarian response to controlled ovarian stimulation (COS). DESIGN: Genetic association study using both single-gene and combined analyses of women with sequence variants undergoing in vitro fertilization treatment. SETTING: Academic research institute hospital. PATIENT(S): Seven hundred and five women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (FSH). INTERVENTION(S): Peripheral blood extraction, DNA purification, and FSHR c.919G>A (rs6165, p.Thr307Ala) and CYP19A1 c.*19C>T (rs10046) sequence variants analyses. MAIN OUTCOME MEASURE(S): Single-gene statistical analysis and combined statistical analysis with the SPSS17.0 software; FSHR c.919G>A and CYP19A1 c.*19C>T sequence variant genotypes and clinical parameters related to the COS response as oocyte retrieval and hormone levels, doses of exogenous FSH. RESULT(S): Women with genotype Ala/Ala at FSHR position 307 had higher basal levels of FSH and were more likely to have a low ovarian response compared with other genotypes. Women with genotype TT at CYP19A1 yielded fewer oocytes after ovarian stimulation. The combined analysis of these two sequence variants revealed that these two single-nucleotide variants have a synergistic effect in conferring the risk of a low ovarian response. CONCLUSION(S): Our results support an association of sequence variants in the genes that participate in estrogen synthesis, notably the FSHR and CYP19A1 genes, with the outcome of COS.
OBJECTIVE: To examine whether sequence variants within the FSHR and CYP19A1 genes are related to the ovarian response to controlled ovarian stimulation (COS). DESIGN: Genetic association study using both single-gene and combined analyses of women with sequence variants undergoing in vitro fertilization treatment. SETTING: Academic research institute hospital. PATIENT(S): Seven hundred and five women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (FSH). INTERVENTION(S): Peripheral blood extraction, DNA purification, and FSHR c.919G>A (rs6165, p.Thr307Ala) and CYP19A1 c.*19C>T (rs10046) sequence variants analyses. MAIN OUTCOME MEASURE(S): Single-gene statistical analysis and combined statistical analysis with the SPSS17.0 software; FSHR c.919G>A and CYP19A1 c.*19C>T sequence variant genotypes and clinical parameters related to the COS response as oocyte retrieval and hormone levels, doses of exogenous FSH. RESULT(S): Women with genotype Ala/Ala at FSHR position 307 had higher basal levels of FSH and were more likely to have a low ovarian response compared with other genotypes. Women with genotype TT at CYP19A1 yielded fewer oocytes after ovarian stimulation. The combined analysis of these two sequence variants revealed that these two single-nucleotide variants have a synergistic effect in conferring the risk of a low ovarian response. CONCLUSION(S): Our results support an association of sequence variants in the genes that participate in estrogen synthesis, notably the FSHR and CYP19A1 genes, with the outcome of COS.
Authors: Shun-Long Weng; Shu-Ling Tzeng; Chun-I Lee; Chung-Hsien Liu; Chun-Chia Huang; Shun-Fa Yang; Maw-Sheng Lee; Tsung-Hsien Lee Journal: Int J Environ Res Public Health Date: 2021-06-30 Impact factor: 3.390
Authors: Aleksandra V Moiseeva; Varvara A Kudryavtseva; Vladimir N Nikolenko; Marine M Gevorgyan; Ara L Unanyan; Anastassia A Bakhmet; Mikhail Y Sinelnikov Journal: J Ovarian Res Date: 2021-08-06 Impact factor: 4.234