Chuan Huang1, Lin Lei2, Hui-Lan Wu1, Run-Xin Gan1, Xiao-Bo Yuan2, Li-Qing Fan3, Wen-Bing Zhu4. 1. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China. 2. Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China. 3. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China; Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China. 4. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China; Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China. Electronic address: zhuwenbing0971@sina.com.
Abstract
OBJECTIVE: To evaluate the affect of the duration of donor sperm storage on pregnancy success among women undergoing assisted reproduction. DESIGN: Retrospective cross-sectional study. SETTING: Sperm bank. PATIENT(S): A total of 119,558 specimens retrieved using a clinical information database of young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank of China from 2001 to 2016. INTERVENTION(S): Analysis of semen samples and clinical outcomes after semen use. MAIN OUTCOME MEASURE(S): Clinical information included semen parameters before and after freezing, clinical pregnancy, abortion and live birth rates after semen use. RESULT(S): The sperm's frozen-thaw survival rate decreased from 85.72% to 73.98% after 15 years of cryopreservation (P<.01). The clinical pregnancy rate of women undergoing artificial insemination with donor sperm was 23.09%, 22.36% and 22.32%, the clinical abortion rate was 10.06%, 10.02% and 12.00% and the live birth rate was 82.17%, 80.21% and 80.00% in the groups with 0.5-5, 6-10 and 11-15 storage years, respectively. The clinical pregnancy rate of women undergoing in vitro fertilization was 64.29%, 64.94% and 53.48%, the clinical abortion rate was 12.26%, 11.38% and 17.39% and the live birth rate was 81.63%, 79.11% and 73.91%, in the groups with 0.5-5, 6-10 and 11-15 years, respectively. CONCLUSION(S): The long-term cryostorage of semen in a human sperm bank does not affect clinical outcomes. However, cryopreservation longer than 5 years negatively influenced the quality of frozen-thawed donor sperm samples.
OBJECTIVE: To evaluate the affect of the duration of donor sperm storage on pregnancy success among women undergoing assisted reproduction. DESIGN: Retrospective cross-sectional study. SETTING: Sperm bank. PATIENT(S): A total of 119,558 specimens retrieved using a clinical information database of young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank of China from 2001 to 2016. INTERVENTION(S): Analysis of semen samples and clinical outcomes after semen use. MAIN OUTCOME MEASURE(S): Clinical information included semen parameters before and after freezing, clinical pregnancy, abortion and live birth rates after semen use. RESULT(S): The sperm's frozen-thaw survival rate decreased from 85.72% to 73.98% after 15 years of cryopreservation (P<.01). The clinical pregnancy rate of women undergoing artificial insemination with donor sperm was 23.09%, 22.36% and 22.32%, the clinical abortion rate was 10.06%, 10.02% and 12.00% and the live birth rate was 82.17%, 80.21% and 80.00% in the groups with 0.5-5, 6-10 and 11-15 storage years, respectively. The clinical pregnancy rate of women undergoing in vitro fertilization was 64.29%, 64.94% and 53.48%, the clinical abortion rate was 12.26%, 11.38% and 17.39% and the live birth rate was 81.63%, 79.11% and 73.91%, in the groups with 0.5-5, 6-10 and 11-15 years, respectively. CONCLUSION(S): The long-term cryostorage of semen in a human sperm bank does not affect clinical outcomes. However, cryopreservation longer than 5 years negatively influenced the quality of frozen-thawed donor sperm samples.
Authors: Millissia Ben Maamar; Eric Nilsson; Jennifer L M Thorson; Daniel Beck; Michael K Skinner Journal: Reprod Toxicol Date: 2020-09-06 Impact factor: 3.143
Authors: L M Ataman; M M Laronda; M Gowett; K Trotter; H Anvari; F Fei; A Ingram; M Minette; C Suebthawinkul; Z Taghvaei; M Torres-Vélez; K Velez; S K Adiga; A Anazodo; L Appiah; M T Bourlon; N Daniels; M M Dolmans; C Finlayson; R B Gilchrist; V Gomez-Lobo; E Greenblatt; J A Halpern; K Hutt; E K Johnson; K Kawamura; M Khrouf; D Kimelman; S Kristensen; R T Mitchell; M B Moravek; L Nahata; K E Orwig; M E Pavone; D Pépin; R Pesce; G P Quinn; M P Rosen; E Rowell; K Smith; C Venter; S Whiteside; S Xiao; M Zelinski; K N Goldman; T K Woodruff; F E Duncan Journal: J Assist Reprod Genet Date: 2022-07-23 Impact factor: 3.357