G Royer1, F Fourreau1, B Boulanger2, M Mercier-Darty3, D Ducellier1, F Cizeau1, A Potron4, I Podglajen5, N Mongardon6, J-W Decousser7. 1. Department of Bacteriology and Infection Control, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France. 2. Service d'anesthésie-réanimation chirurgicale, unité d'anesthésie-réanimation chirurgicale cardio-vasculaire, DHU A-TVB, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France. 3. Department of Bacteriology and Infection Control, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France; Next Generation Sequencing platform, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France. 4. Centre National de Référence de la résistance aux antibiotiques, Centre Hospitalier Universitaire de Besançon, UMR6249 CNRS Chrono-Environnement, Université de Franche-Comté, Besançon, France. 5. Department of Bacteriology, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France. 6. Service d'anesthésie-réanimation chirurgicale, unité d'anesthésie-réanimation chirurgicale cardio-vasculaire, DHU A-TVB, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France; INSERM, U955, Equipe 3 'Stratégies pharmacologiques et thérapeutiques expérimentales des insuffisances cardiaques et coronaires', Institut Mondor de Recherche Biomédicale, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France. 7. Department of Bacteriology and Infection Control, University Hospital Henri Mondor, Assistance Publique - Hôpitaux de Paris, Créteil, France; EA 7380 Dynamyc Université Paris - Est Créteil (UPEC), Ecole nationale vétérinaire d'Alfort (EnvA), Faculté de Médecine de Créteil, Créteil, France. Electronic address: jean-winoc.decousser@aphp.fr.
Abstract
BACKGROUND: Pseudomonas aeruginosa is a major bacterial pathogen responsible for hospital-acquired infections. Although its epidemiology is considered as non-clonal, certain international high-risk multidrug-resistant clones have been recognized. AIM: From the first report of an intra-hospital outbreak due to an SHV2a-producing P. aeruginosa strain, to describe the emergence of a new ST235-specific lineage harbouring this rare extended-spectrum β-lactamase (ESBL). METHODS: Between May and October 2018, four patients hospitalized in the cardiovascular intensive care unit of a French teaching hospital were infected by a multidrug-resistant P. aeruginosa isolate. Serotype and antimicrobial susceptibility were tested; multi-locus sequence type (MLST), core genome MLST, and resistome were determined through whole genome sequencing. A phylogenetic analysis based on single nucleotide polymorphism was performed using available ST235 genomes. FINDINGS: The four strains were susceptible to colistin, ciprofloxacin, ceftazidime-avibactam, and ceftolozane-tazobactam. blaSHV2a was identified in each genome of this ST235-O11 serotype cluster that showed an identical cgMLST profile (0-2 out of 4162 different alleles). The phylogenic analysis of 162 ST235 genomes showed that only four other strains harboured a blaSHV2a, originating from France and USA, clustering together although being different from the outbreak strains. CONCLUSIONS: Among the ST235 P. aeruginosa strains, a sub-lineage sharing a common genetic background and harbouring the blaSHV2a ESBL seems to emerge from different locations, yielding secondary local outbreaks.
BACKGROUND:Pseudomonas aeruginosa is a major bacterial pathogen responsible for hospital-acquired infections. Although its epidemiology is considered as non-clonal, certain international high-risk multidrug-resistant clones have been recognized. AIM: From the first report of an intra-hospital outbreak due to an SHV2a-producing P. aeruginosa strain, to describe the emergence of a new ST235-specific lineage harbouring this rare extended-spectrum β-lactamase (ESBL). METHODS: Between May and October 2018, four patients hospitalized in the cardiovascular intensive care unit of a French teaching hospital were infected by a multidrug-resistant P. aeruginosa isolate. Serotype and antimicrobial susceptibility were tested; multi-locus sequence type (MLST), core genome MLST, and resistome were determined through whole genome sequencing. A phylogenetic analysis based on single nucleotide polymorphism was performed using available ST235 genomes. FINDINGS: The four strains were susceptible to colistin, ciprofloxacin, ceftazidime-avibactam, and ceftolozane-tazobactam. blaSHV2a was identified in each genome of this ST235-O11 serotype cluster that showed an identical cgMLST profile (0-2 out of 4162 different alleles). The phylogenic analysis of 162 ST235 genomes showed that only four other strains harboured a blaSHV2a, originating from France and USA, clustering together although being different from the outbreak strains. CONCLUSIONS: Among the ST235 P. aeruginosa strains, a sub-lineage sharing a common genetic background and harbouring the blaSHV2a ESBL seems to emerge from different locations, yielding secondary local outbreaks.
Authors: Kevin Y H Liang; Fabini D Orata; Mohammad Tarequl Islam; Tania Nasreen; Munirul Alam; Cheryl L Tarr; Yann F Boucher Journal: J Bacteriol Date: 2020-11-19 Impact factor: 3.490
Authors: Emanuela Roscetto; Marco Masi; Matilde Esposito; Roberta Di Lecce; Antonella Delicato; Lucia Maddau; Viola Calabrò; Antonio Evidente; Maria Rosaria Catania Journal: Toxins (Basel) Date: 2020-07-08 Impact factor: 4.546