Thomas Burchard1, Lamprini Karygianni2, Elmar Hellwig3, Marie Follo4, Thomas Wrbas3, Annette Wittmer5, Kirstin Vach6, Ali Al-Ahmad3. 1. Department of Prosthetic Dentistry, Center for Dental Medicine, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. 2. Clinic of Preventive Dentistry, Periodontology & Cariology, Center of Dental Medicine, University of Zurich, Switzerland. 3. Department of Operative Dentistry & Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. 4. Department of Hematology, Oncology & Stem Cell Transplantation, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. 5. Department of Hygiene & Microbiology, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. 6. Department of Medical Biometry & Statistics, Faculty of Medicine, University of Freiburg, Germany.
Abstract
Aim: To investigate the antimicrobial photodynamic therapy (aPDT) of visible light and water-filtered infrared A radiation in combination with indocyanine green (ICG) on planktonic oral microorganisms as well as on oral biofilm. Methods: The irradiation was conducted for 5 min in combination with ICG. Treatment with chlorhexidine served as a positive control. The number of colony forming units and bacterial vitality were quantified. Results: All tested bacterial strains and salivary bacteria were killed at a level of 3log10. The colony forming units of the initial mature oral biofilms were strongly reduced. The high bactericidal effect of aPDT was confirmed by live/dead staining. Conclusion: The aPDT using visible light and water-filtered infrared A radiation and ICG has the potential to treat periodontitis and peri-implantitis.
Aim: To investigate the antimicrobial photodynamic therapy (aPDT) of visible light and water-filtered infrared A radiation in combination with indocyanine green (ICG) on planktonic oral microorganisms as well as on oral biofilm. Methods: The irradiation was conducted for 5 min in combination with ICG. Treatment with chlorhexidine served as a positive control. The number of colony forming units and bacterial vitality were quantified. Results: All tested bacterial strains and salivary bacteria were killed at a level of 3log10. The colony forming units of the initial mature oral biofilms were strongly reduced. The high bactericidal effect of aPDT was confirmed by live/dead staining. Conclusion: The aPDT using visible light and water-filtered infrared A radiation and ICG has the potential to treat periodontitis and peri-implantitis.
Entities:
Keywords:
ICG; VIS+wIRA; aPDT; antimicrobial photodynamic therapy; indocyanine green; oral biofilm; visible light and water-filtered infrared A