N Zaatout1, A Ayachi2, M Kecha1, K Kadlec3. 1. Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia, Algeria. 2. Institute of Veterinary and Agricultural Sciences, University of Batna, Batna, Algeria. 3. Dairy Herd Consulting and Research Company (MBFG), Wunstorf, Germany.
Abstract
AIMS: This study was conducted to determine the occurrence of staphylococci from cows with subclinical mastitis from independent herds in Algeria, and to characterize Staphylococcus aureus isolates. METHODS AND RESULTS: Quarter milk samples were collected separately, somatic cells were counted and samples with more than 200 000 somatic cells per ml were cultured on blood agar. Staphylococci isolates were identified by routine diagnostics, and S. aureus isolates were tested for antibiotic susceptibility by disk diffusion and microdilution. Congo red agar was used to detect biofilm formation and capsule synthesis was detected on serum soft agar (SSA). The S. aureus isolates were characterized by spa typing. DNA microarray analysis was performed to detect resistance and virulence genes. Overall, 40·0% (167/418) of the cows suffered from mastitis. In 63·5% (106/167) of the cows staphylococci were identified. Nine of the 106 Staphylococcus isolates (8·5%) were S. aureus. The coagulase-negative staphylococci belonged to 14 species. All S. aureus isolates were multiresistant and biofilm forming, with 66·67% of them showing diffuse colonies on SSA and belonged to CC97-agrI-cap5. Biofilm genes (icaA/C/D), 13 genes encoding for adhesion, six genes encoding proteases, 11 genes encoding superantigen like toxins were found. Genes conferring resistance to tetracycline (tet(K)), penicillin (blaZ/I/R) and macrolide-lincosamide-streptogramin B (erm(B), erm(A)) were also detected in the S. aureus from this study. CONCLUSIONS: The current investigation provides a detailed molecular and biofilm formation ability of S. aureus involved in subclinical mastitis in Algeria and shows the wide distribution of adhesion and enterotoxin(-like) genes among S. aureus responsible for causing subclinical bovine mastitis. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings are valuable in tracking the evolution and genomic variation of S. aureus from bovine origin.
AIMS: This study was conducted to determine the occurrence of staphylococci from cows with subclinical mastitis from independent herds in Algeria, and to characterize Staphylococcus aureus isolates. METHODS AND RESULTS: Quarter milk samples were collected separately, somatic cells were counted and samples with more than 200 000 somatic cells per ml were cultured on blood agar. Staphylococci isolates were identified by routine diagnostics, and S. aureus isolates were tested for antibiotic susceptibility by disk diffusion and microdilution. Congo red agar was used to detect biofilm formation and capsule synthesis was detected on serum soft agar (SSA). The S. aureus isolates were characterized by spa typing. DNA microarray analysis was performed to detect resistance and virulence genes. Overall, 40·0% (167/418) of the cows suffered from mastitis. In 63·5% (106/167) of the cows staphylococci were identified. Nine of the 106 Staphylococcus isolates (8·5%) were S. aureus. The coagulase-negative staphylococci belonged to 14 species. All S. aureus isolates were multiresistant and biofilm forming, with 66·67% of them showing diffuse colonies on SSA and belonged to CC97-agrI-cap5. Biofilm genes (icaA/C/D), 13 genes encoding for adhesion, six genes encoding proteases, 11 genes encoding superantigen like toxins were found. Genes conferring resistance to tetracycline (tet(K)), penicillin (blaZ/I/R) and macrolide-lincosamide-streptogramin B (erm(B), erm(A)) were also detected in the S. aureus from this study. CONCLUSIONS: The current investigation provides a detailed molecular and biofilm formation ability of S. aureus involved in subclinical mastitis in Algeria and shows the wide distribution of adhesion and enterotoxin(-like) genes among S. aureus responsible for causing subclinical bovinemastitis. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings are valuable in tracking the evolution and genomic variation of S. aureus from bovine origin.
Authors: Geziella Áurea Aparecida Damasceno Souza; Anna Christina de Almeida; Mauro Aparecido de Sousa Xavier; Lívia Mara Vitorino da Silva; Cintya Neves Sousa; Demerson Arruda Sanglard; Alessandra Rejane Ericsson de Oliveira Xavier Journal: Vet World Date: 2019-12-11
Authors: Bruno Campos; Amy C Pickering; Lis Souza Rocha; Ananda Pereira Aguilar; Mary Hellen Fabres-Klein; Tiago Antônio de Oliveira Mendes; J Ross Fitzgerald; Andrea de Oliveira Barros Ribon Journal: BMC Vet Res Date: 2022-03-24 Impact factor: 2.741