Mark Jakob1, Lena M Mattes2, Stefan Küffer3, Kristian Unger4,5, Julia Hess4,5, Mattis Bertlich1, Frank Haubner1, Friedrich Ihler1,6, Martin Canis1, Bernhard G Weiss1, Julia Kitz3,6. 1. Department of Otorhinolaryngology, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany. 2. Department of Otorhinolaryngology, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany. 3. Institute of Pathology, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany. 4. Clinical Cooperation Group "Personalized Radiotherapy in Head and Neck Cancer", Helmholtz Zentrum München, Research Center for Environmental Health (GmbH), Munich, Germany. 5. Research Unit Radiation Cytogenetics, Helmholtz Zentrum München, Research Center for Environmental Health (GmbH), Munich, Germany. 6. German Center for Vertigo and Balance Disorders (DSGZ), Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany.
Abstract
BACKGROUND: MicroRNAs (miRNA) recently evolved as potential cancer biomarkers. Therefore, the aim of the present study was to evaluate the prognostic impact of eight miRNAs connected to oral squamous cell carcinoma (OSCC). METHOD: Expression levels of hsa-mir-21-5p, hsa-mir-29b-3p, hsa-mir-31-5p, hsa-mir-99a-5p, hsa-mir-99b-3p, hsa-mir-100-5p, hsa-mir-143-3p and hsa-mir-155-5p were analyzed in tumor tissue (n = 36) and healthy oral mucosal tissue (n = 17) and correlated with clinical variables. Results of the study cohort were validated in an OSCC cohort of The Cancer Genome Atlas. RESULTS: Increased hsa-mir-99b-3p expression level showed a tendency toward advanced tumor stages, and high levels of hsa-mir-100-5p expression were associated with extracapsular extension. While a high expression level of hsa-mir-99b-3p was associated with better survival, a high expression level of hsa-mir-100-5p was correlated with a poorer survival in the study cohort. CONCLUSION: Our results indicate that hsa-mir-99b-3p and hsa-mir-100-5p may serve as novel prognostic biomarkers in OSCC.
BACKGROUND: MicroRNAs (miRNA) recently evolved as potential cancer biomarkers. Therefore, the aim of the present study was to evaluate the prognostic impact of eight miRNAs connected to oral squamous cell carcinoma (OSCC). METHOD: Expression levels of hsa-mir-21-5p, hsa-mir-29b-3p, hsa-mir-31-5p, hsa-mir-99a-5p, hsa-mir-99b-3p, hsa-mir-100-5p, hsa-mir-143-3p and hsa-mir-155-5p were analyzed in tumor tissue (n = 36) and healthy oral mucosal tissue (n = 17) and correlated with clinical variables. Results of the study cohort were validated in an OSCC cohort of The Cancer Genome Atlas. RESULTS: Increased hsa-mir-99b-3p expression level showed a tendency toward advanced tumor stages, and high levels of hsa-mir-100-5p expression were associated with extracapsular extension. While a high expression level of hsa-mir-99b-3p was associated with better survival, a high expression level of hsa-mir-100-5p was correlated with a poorer survival in the study cohort. CONCLUSION: Our results indicate that hsa-mir-99b-3p and hsa-mir-100-5p may serve as novel prognostic biomarkers in OSCC.
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Lucia La Femina; Alessandra Campobasso; Angela Pia Cazzolla; Michele Di Cosola; Khrystyna Zhurakivska; Stefania Cantore; Andrea Ballini; Lorenzo Lo Muzio; Giuseppe Troiano Journal: Biology (Basel) Date: 2022-04-24
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Mario Alovisi; Riccardo Aiuto; Daniele Garcovich; Vito Crincoli; Luigi Laino; Angela Pia Cazzolla; Giorgia Apollonia Caloro; Michele Di Cosola; Andrea Ballini; Lorenzo Lo Muzio; Giuseppe Troiano Journal: Int J Environ Res Public Health Date: 2022-04-27 Impact factor: 4.614
Authors: Óscar Rapado-González; Rafael López-López; José Luis López-Cedrún; Gabriel Triana-Martínez; Laura Muinelo-Romay; María Mercedes Suárez-Cunqueiro Journal: Cells Date: 2019-12-17 Impact factor: 6.600
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Mario Alovisi; Giuseppe Troiano; Riccardo Aiuto; Daniele Garcovich; Vito Crincoli; Luigi Laino; Angela Pia Cazzolla; Giorgia Apollonia Caloro; Michele Di Cosola; Lorenzo Lo Muzio Journal: Int J Environ Res Public Health Date: 2022-03-14 Impact factor: 3.390