Literature DB >> 3135282

Direct fluorescence localization of an endogenous N-acetyl-glucosamine-specific lectin in the thyroid gland.

C Alquier1, R Miquelis, M Monsigny.   

Abstract

A sugar-binding protein, or endogenous lectin, was localized on fixed and paraffin-embedded thyroid sections by means of fluorescein-labelled neoglycoproteins. Fluorescence microscopy showed the binding of this lectin to be dependent on calcium ions and acidic pH and indicated sugar specificity for GlcNAc moieties only. In human, porcine and murine thyrocytes, specific binding was observed mainly on subcellular organelles. Conversely, in rabbit thyrocytes, specific labelling was seen mostly at the apical cell surface facing the follicular lumen. The possibility that this novel endogenous lectin is involved in the Tg metabolism is considered.

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Year:  1988        PMID： 3135282     DOI： 10.1007/bf00489920

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  35 in total

1.  Detection of sugar-binding proteins in membrane-depleted nuclei.

Authors:  A P Seve; J Hubert; D Bouvier; M Bouteille; C Maintier; M Monsigny
Journal:  Exp Cell Res       Date:  1985-04       Impact factor: 3.905

Review 2.  Soluble lectins: a new class of extracellular proteins.

Authors:  S H Barondes
Journal:  Science       Date:  1984-03-23       Impact factor: 47.728

3.  The pathway of the asialoglycoprotein-ligand during receptor-mediated endocytosis: a morphological study with colloidal gold/ligand in the human hepatoma cell line, Hep G2.

Authors:  H J Geuze; J W Slot; G J Strous; A L Schwartz
Journal:  Eur J Cell Biol       Date:  1983-11       Impact factor: 4.492

4.  A simple method of reducing the fading of immunofluorescence during microscopy.

Authors:  G D Johnson; G M Nogueira Araujo
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

5.  Endocytosis via galactose receptors in vivo. Ligand size directs uptake by hepatocytes and/or liver macrophages.

Authors:  J Schlepper-Schäfer; D Hülsmann; A Djovkar; H E Meyer; L Herbertz; H Kolb; V Kolb-Bachofen
Journal:  Exp Cell Res       Date:  1986-08       Impact factor: 3.905

6.  Immunocytochemical localization of the mannose receptor on ultrathin cryosections of chicken macrophages.

Authors:  G Rossi; S Himmelhoch
Journal:  Eur J Cell Biol       Date:  1985-09       Impact factor: 4.492

7.  Sugar-specific endocytosis of glycoproteins by Lewis lung carcinoma cells.

Authors:  A C Roche; M Barzilay; P Midoux; S Junqua; N Sharon; M Monsigny
Journal:  J Cell Biochem       Date:  1983       Impact factor: 4.429

8.  Uptake of neoglycoproteins via membrane lectin(s) of L1210 cells evidenced by quantitative flow cytofluorometry and drug targeting.

Authors:  M Monsigny; A C Roche; P Midoux
Journal:  Biol Cell       Date:  1984       Impact factor: 4.458

9.  Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double-label immunoelectron microscopy during receptor-mediated endocytosis.

Authors:  H J Geuze; J W Slot; G J Strous; H F Lodish; A L Schwartz
Journal:  Cell       Date:  1983-01       Impact factor: 41.582

10.  Surface Plasmodium sugar-binding components evidenced by fluorescent neoglycoproteins.

Authors:  J Schrevel; M Philippe; F Bernard; M Monsigny
Journal:  Biol Cell       Date:  1986       Impact factor: 4.458
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  2 in total

1.  Phenotypic changes of the thyrocyte membrane in papillary thyroid carcinoma. A three-dimensional study.

Authors:  A Crescenzi; S Taccogna; L Turrini; D Cicciarella Modica; E Papini; A Gallo; L Guidobaldi; G Zelano; C Giannakakis; F Nardi
Journal:  J Endocrinol Invest       Date:  2010-09-17       Impact factor: 4.256

2.  Intracellular routing of GLcNAc-bearing molecules in thyrocytes: selective recycling through the Golgi apparatus.

Authors:  R Miquelis; J Courageot; A Jacq; O Blanck; C Perrin; P Bastiani
Journal:  J Cell Biol       Date:  1993-12       Impact factor: 10.539
  2 in total

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