Doğukan Özdemir1, İbrahim Ağrı2, Mustafa Bakirtaş3, Arzu Ağrı4, Dursun Mehmet Mehel5, Mehmet Çelebi5, Abdulkadir Özgür5. 1. Department of Otorhinolaryngology, University of Health Sciences, Samsun, Turkey. Electronic address: drdogukan@hotmail.com. 2. Department of Otorhinolaryngology, Samsun Training and Research Hospital, Samsun, Turkey. 3. Department of Pathology, Samsun Training and Research Hospital, Samsun, Turkey. 4. Department of Pharmacology, University of Ondokuz Mayıs, Samsun, Turkey. 5. Department of Otorhinolaryngology, University of Health Sciences, Samsun, Turkey.
Abstract
OBJECTIVES: Myringosclerosis commonly occurs as a long-term complication of ventilation during the treatment of otitis media. We aimed to determine the effects of rosmarinic acid as an antioxidant on experimentally induced myringosclerosis. METHODS: Twenty-four male Sprague-Dawley rats, weighing 250-300 g, were unilaterally myringotomized and randomly separated into three groups. Group 1 received no treatment (control group) (n = 8); Group 2 received topical rosmarinic acid (n = 8); Group 3 received oral rosmarinic acid (n = 8). On the twenty-first day, the right ears were examined by otomicroscope and findings of myringosclerosis were recorded. Finally, all of the rats were euthanized and the tympanic membrane (TM) thickness and the severity of middle ear mucosal inflammation were evaluated histopathologically. RESULTS: The myringosclerosis severity, TM thickness, and inflammation scores were found to be significantly higher in the control group than in the topical and systemic treatment groups (p < 0.05). There were no statistically significant differences in terms of TM thickness and inflammation scores between the topical and systemic treatment groups (p > 0.05). While moderate and severe myringosclerosis were higher in the control group, mild myringosclerosis was found to be higher in both treatment groups. CONCLUSION: The local and oral administration of rosmarinic acid suppressed inflammation, reduced TM thickness, and prevented the development of myringosclerosis in myringotomized rats.
OBJECTIVES:Myringosclerosis commonly occurs as a long-term complication of ventilation during the treatment of otitis media. We aimed to determine the effects of rosmarinic acid as an antioxidant on experimentally induced myringosclerosis. METHODS: Twenty-four male Sprague-Dawley rats, weighing 250-300 g, were unilaterally myringotomized and randomly separated into three groups. Group 1 received no treatment (control group) (n = 8); Group 2 received topical rosmarinic acid (n = 8); Group 3 received oral rosmarinic acid (n = 8). On the twenty-first day, the right ears were examined by otomicroscope and findings of myringosclerosis were recorded. Finally, all of the rats were euthanized and the tympanic membrane (TM) thickness and the severity of middle ear mucosal inflammation were evaluated histopathologically. RESULTS: The myringosclerosis severity, TM thickness, and inflammation scores were found to be significantly higher in the control group than in the topical and systemic treatment groups (p < 0.05). There were no statistically significant differences in terms of TM thickness and inflammation scores between the topical and systemic treatment groups (p > 0.05). While moderate and severe myringosclerosis were higher in the control group, mild myringosclerosis was found to be higher in both treatment groups. CONCLUSION: The local and oral administration of rosmarinic acid suppressed inflammation, reduced TM thickness, and prevented the development of myringosclerosis in myringotomized rats.
Authors: Oğuz Kadir Eğilmez; Mehmet Güven; Sena Genç Elden; Özcan Budak; Hüseyin Çakıroğlu; Halil Elden; Ebru Mihriban Güven Journal: Turk Arch Otorhinolaryngol Date: 2021-07-30