Literature DB >> 3135125

Evaluation of a dual-antigen ELISA test for the serodiagnosis of tuberculosis.

S F Raheman, S Wagner, H Mauch, N D Vasudeva, D L Ingole.   

Abstract

Two antigen preparations from Mycobacterium tuberculosis and M. bovis, respectively, were used in an enzyme-linked immunosorbent assay (ELISA) to quantitate the level of IgG antibodies in samples of 169 sera obtained in India from 75 patients with pulmonary tuberculosis and from 94 controls. The results of the two ELISA tests were integrated using defined criteria. All the controls were correctly classified by this dual-ELISA approach and only two patients were categorized as false negatives. The method has a specificity of 1.00 and a sensitivity of 0.974, while the gain in the certainty of diagnosis was 1.974, only slightly less than the ideal value of 2.00.

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Year:  1988        PMID： 3135125      PMCID： PMC2491040     

Source DB:  PubMed          Journal:  Bull World Health Organ        ISSN： 0042-9686            Impact factor:   9.408


  6 in total

1.  ELISA of IgG antibody to M. tuberculosis antigen 5 for serodiagnosis of tuberculosis.

Authors:  T M Daniel; R G Benjamin; S M Debanne; Y Ma; E A Balestrino
Journal:  Indian J Pediatr       Date:  1985 Jul-Aug       Impact factor: 1.967

2.  The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA).

Authors:  E Nassau; E R Parsons; G D Johnson
Journal:  Tubercle       Date:  1976-03

3.  Measures of gain in certainty from a diagnostic test.

Authors:  F A Connell; T D Koepsell
Journal:  Am J Epidemiol       Date:  1985-05       Impact factor: 4.897

4.  Preparation and properties of antigen 60 from Mycobacterium bovis BCG.

Authors:  C Cocito; F Vanlinden
Journal:  Clin Exp Immunol       Date:  1986-11       Impact factor: 4.330

5.  Enzyme-linked immunosorbent assay (ELISA): a study of antibodies to Mycobacterium tuberculosis in the IgG, IgA and IgM classes in tuberculosis, sarcoidosis and Crohn's disease.

Authors:  J M Grange; J Gibson; E Nassau; T Kardjito
Journal:  Tubercle       Date:  1980-09

6.  Analysis of a solid-phase radioimmunoassay for antibodies to cytoplasmic antigen fractions of Candida albicans.

Authors:  H Mauch; J Brombach
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303
  6 in total
  6 in total

1.  Public health application of a serological test for tuberculosis: study of the incidence of inapparent infections among the employees of an Alsatian supermarket.

Authors:  C Wirrmann
Journal:  Eur J Epidemiol       Date:  1990-09       Impact factor: 8.082

2.  Evaluation of the avidity of IgG anti-mycobacterial antibodies in tuberculous patients serum by an A-60 immunoassay.

Authors:  R F Maes
Journal:  Eur J Epidemiol       Date:  1991-03       Impact factor: 8.082

3.  Serological comparison of purified antigens 60 and 85A (P32) of Mycobacterium bovis BCG, and purified protein derivative, in active pulmonary tuberculosis.

Authors:  M Turneer; E Van Nerom
Journal:  Eur J Epidemiol       Date:  1993-09       Impact factor: 8.082

4.  Competitive and indirect enzyme-linked immunosorbent assays for Mycobacterium bovis infections based on MPB70 and lipoarabinomannan antigens.

Authors:  E A Sugden; K Stilwell; E B Rohonczy; P Martineau
Journal:  Can J Vet Res       Date:  1997-01       Impact factor: 1.310

5.  Clinical usefulness of serological measurements obtained by antigen 60 in mycobacterial infections: development of a new concept.

Authors:  R Maes
Journal:  Klin Wochenschr       Date:  1991-10-02

6.  Serological response of tuberculosis patients to antigen 60 of BCG.

Authors:  M J Gevaudan; C Bollet; D Charpin; M N Mallet; P De Micco
Journal:  Eur J Epidemiol       Date:  1992-09       Impact factor: 8.082
  6 in total

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