Literature DB >> 3134953

Molecular cloning and overexpression of the glucosamine synthetase gene from Escherichia coli.

S Dutka-Malen1, P Mazodier, B Badet.   

Abstract

A recombinant plasmid carrying a 4.6 kg restriction endonuclease NcoI-ClaI fragment of genomic DNA from Escherichia coli K12 was constructed. This plasmid complements the glmS mutation. Subcloning into pUC18 gave plasmid pGM10 encoding the structural gene of glucosamine synthetase, as judged by overexpression of enzyme activity and the isolation in high yield of the pure protein.

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Year:  1988        PMID： 3134953     DOI： 10.1016/0300-9084(88)90073-9

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  15 in total

1.  Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli.

Authors:  J Plumbridge; E Vimr
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

2.  Probing the role of cysteine residues in glucosamine-1-phosphate acetyltransferase activity of the bifunctional GlmU protein from Escherichia coli: site-directed mutagenesis and characterization of the mutant enzymes.

Authors:  F Pompeo; J van Heijenoort; D Mengin-Lecreulx
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

3.  Autophosphorylation of phosphoglucosamine mutase from Escherichia coli.

Authors:  L Jolly; F Pompeo; J van Heijenoort; F Fassy; D Mengin-Lecreulx
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

4.  Use of the plant defense protein osmotin to identify Fusarium oxysporum genes that control cell wall properties.

Authors:  Hyeseung Lee; Barbara Damsz; Charles P Woloshuk; Ray A Bressan; Meena L Narasimhan
Journal:  Eukaryot Cell       Date:  2010-02-26

5.  Complete nucleotide sequence of the 27-kilobase virulence related locus (vrl) of Dichelobacter nodosus: evidence for extrachromosomal origin.

Authors:  S J Billington; A S Huggins; P A Johanesen; P K Crellin; J K Cheung; M E Katz; C L Wright; V Haring; J I Rood
Journal:  Infect Immun       Date:  1999-03       Impact factor: 3.441

6.  Identification of the glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli.

Authors:  D Mengin-Lecreulx; J van Heijenoort
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

7.  Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis.

Authors:  D Mengin-Lecreulx; J van Heijenoort
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

8.  Acetate-mediated pH-stat fed-batch cultivation of transconjugant Enterobacter sp. BL-2S over-expressing glmS gene for excretive production of microbial polyglucosamine PGB-1.

Authors:  Mi-Kyung Son; Soo-Jung Hong; Yong-Hyun Lee
Journal:  J Ind Microbiol Biotechnol       Date:  2007-10-02       Impact factor: 3.346

9.  Isolation and characterization of the GFA1 gene encoding the glutamine:fructose-6-phosphate amidotransferase of Candida albicans.

Authors:  R J Smith; S Milewski; A J Brown; G W Gooday
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490

10.  N-acetylglucosamine (GlcNAc) functions in cell signaling.

Authors:  James B Konopka
Journal:  Scientifica (Cairo)       Date:  2012-10-23
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