| Literature DB >> 31347711 |
Atang Bulane1, Dominique Goedhals1,2, Riaz Y Seedat3,4, Jacqueline Goedhals5,6, Felicity Burt1,2.
Abstract
Human papillomaviruses (HPVs) have been associated with a subset of head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to determine the prevalence of HPV DNA in archived formalin-fixed paraffin-embedded tissue from patients with histologically confirmed HNSCCs in a South African cohort. A nested PCR was used for the detection of HPV DNA targeting the L1 gene. Positive samples were confirmed using an in-house hemi-nested PCR targeting the E6 gene and genotyped by sequence determination of amplicons. HPV DNA was detected in 57/780 (7.3%) samples, with the highest prevalence being in the sinonasal tract (16.0%) and oropharynx (10.8%). HPV16 was the most frequently detected type, being found in 26/57 (45.6%) positive samples. The prevalence of HPV DNA in HNSCCs found in this study was lower than that found in developed countries.Entities:
Keywords: formalin-fixed paraffin-embedded tissues; head, and neck squamous cell carcinomas; human papillomaviruses; hypopharynx carcinoma; larynx carcinoma; nasopharynx carcinoma; oral squamous cell carcinoma; oropharynx carcinoma; prevalence; sinonasal carcinoma
Mesh：
Substances：
Year: 2020 PMID： 31347711 DOI： 10.1002/jmv.25556
Source DB: PubMed Journal: J Med Virol ISSN： 0146-6615 Impact factor: 2.327