AIM: Oral squamous cell carcinoma (OSCC) is the most frequently occurring cancer among head and neck SCC worldwide. The identification of novel effective biomarkers for early detection may greatly improve the survival rate and prognosis of patients with OSCC. This study aimed to identify specific oral microbial profiles associated with OSCC. METHODS: Saliva samples were collected from oral leukoplakia (OLK) and OSCC patients (N = 6 each) and healthy controls (HC; N = 4). Total bacterial genomic DNA was isolated and 16S rRNA gene survey was performed by next-generation sequencing of the V4 region. The relative distribution of abundance for phylogenetic groups was compared among the OSCC and OLK groups. RESULTS: The 448 operational taxonomic units detected from the libraries were classified into 133 genera, 69 families, 41 orders, 26 classes and 12 phyla. The abundance of phyla Bacteroidetes and genus Solobacterium was notably higher in the OSCC group when compared with the OLK group, whereas those of genus Streptococcus was significantly lower in the OSCC group when compared with the OLK. CONCLUSION: These changes in the salivary microbiome may have potential applications as a novel diagnostic tool for the early detection of OSCC.
AIM: Oral squamous cell carcinoma (OSCC) is the most frequently occurring cancer among head and neck SCC worldwide. The identification of novel effective biomarkers for early detection may greatly improve the survival rate and prognosis of patients with OSCC. This study aimed to identify specific oral microbial profiles associated with OSCC. METHODS: Saliva samples were collected from oral leukoplakia (OLK) and OSCC patients (N = 6 each) and healthy controls (HC; N = 4). Total bacterial genomic DNA was isolated and 16S rRNA gene survey was performed by next-generation sequencing of the V4 region. The relative distribution of abundance for phylogenetic groups was compared among the OSCC and OLK groups. RESULTS: The 448 operational taxonomic units detected from the libraries were classified into 133 genera, 69 families, 41 orders, 26 classes and 12 phyla. The abundance of phyla Bacteroidetes and genus Solobacterium was notably higher in the OSCC group when compared with the OLK group, whereas those of genus Streptococcus was significantly lower in the OSCC group when compared with the OLK. CONCLUSION: These changes in the salivary microbiome may have potential applications as a novel diagnostic tool for the early detection of OSCC.
Authors: Rodrigo Alex Arthur; Rafael Dos Santos Bezerra; João Paulo Bianchi Ximenez; Bruna Laís Merlin; Raphael de Andrade Morraye; João Valentini Neto; Natália Melo Nasser Fava; David Livingstone Alves Figueiredo; Carlos Alberto Oliveira de Biagi; Maria Jara Montibeller; Jhefferson Barbosa Guimarães; Ellen Gomes Alves; Monique Schreiner; Tiago Silva da Costa; Charlie Felipe Liberati da Silva; Jessica Moraes Malheiros; Luan Henrique Burda da Silva; Guilherme Taborda Ribas; Daisy Obispo Achallma; Camila Margalho Braga; Karen Flaviane Assis Andrade; Valquiria do Carmo Alves Martins; Glauco Vinícius Nestor Dos Santos; Caroline Fabiane Granatto; Ulisses Costa Terin; Igor Henrique Sanches; Diana Estefania Ramos; Humberto Miguel Garay-Malpartida; Gabriela Marcelino Pereira de Souza; Svetoslav Nanev Slavov; Wilson Araújo Silva Journal: Braz J Microbiol Date: 2021-05-18 Impact factor: 2.476
Authors: Egija Zaura; Vincent Y Pappalardo; Mark J Buijs; Catherine M C Volgenant; Bernd W Brandt Journal: Periodontol 2000 Date: 2020-11-23 Impact factor: 7.589