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Literature DB >> 3134011

Drosophila melanogaster alcohol dehydrogenase. Biochemical properties of the NAD+-plus-acetone-induced isoenzyme conversion.

Abstract

The NAD+ + acetone-induced isoenzyme conversion of the Drosophila melanogaster AdhS alleloenzyme was studied. Absorption and fluorescence spectra as well as electrophoretic and kinetic methods show that the conversion process proceeds through three steps. Initially a binary enzyme-NAD+ complex is formed, followed by a ternary enzyme-NAD+-acetone complex with a KEO,Ac of 1.7 M. The last step is a rate-limiting irreversible process in which NAD+ and acetone are covalently linked to the enzyme. A Vm of 2.4 min-1 was obtained at pH 8.6.

Entities: Chemical Disease Species

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Year: 1988 PMID： 3134011 PMCID： PMC1148987 DOI： 10.1042/bj2510223

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

35 in total

1. The alcohol dehydrogenase alleloenzymes AdhS and AdhF from the fruitfly Drosophila melanogaster: an enzymatic rate assay to determine the active-site concentration.

Authors: J O Winberg; R Hovik; J S McKinley-McKee
Journal: Biochem Genet Date: 1985-04 Impact factor: 1.890

2. Multiple forms of drosophila alcohol dehydrogenase. 3. Conversion of one form to another by nicotinamide adenine dinucleotide or acetone.

Authors: K B Jacobson; J B Murphy; J A Knopp; J R Ortiz
Journal: Arch Biochem Biophys Date: 1972-03 Impact factor: 4.013

3. Isoenzymes of Drosophila alcohol dehydrogenase. I. Isolation and interconversion of different forms.

Authors: K B Jacobson; J B Murphy; F C Hartman
Journal: J Biol Chem Date: 1970-03-10 Impact factor: 5.157

4. Interconversion of isoenzymes of Drosophila alcohol dehydrogenase. II. Physical characterization of the enzyme and its subunits.

Authors: K B Jacobson; P Pfuderer
Journal: J Biol Chem Date: 1970-08-10 Impact factor: 5.157

5. Structural analysis of an electrophoretically cryptic alcohol dehydrogenase variant from an Australian population of Drosophila melanogaster.

Authors: G K Chambers; W G Laver; S Campbell; J B Gibson
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

6. Anion-binding to liver alcohol dehydrogenase, studied by rate of alkylation.

Authors: C H Reynolds; J S McKinley-McKee
Journal: Eur J Biochem Date: 1969-10

7. Alcohol dehydrogenase of Drosophila: interconversion of isoenzymes.

Authors: K B Jacobson
Journal: Science Date: 1968-01-19 Impact factor: 47.728

8. Alcohol dehydrogenase from the fruitfly Drosophila melanogaster. Inhibition studies of the alleloenzymes AdhS and AdhUF.

Authors: J O Winberg; D R Thatcher; J S McKinley-McKee
Journal: Biochim Biophys Acta Date: 1982-05-21

9. Alcohol Dehydrogenase in Drosophila melanogaster: Isozymes and Genetic Variants.

Authors: E H Grell; K B Jacobson; J B Murphy
Journal: Science Date: 1965-07-02 Impact factor: 47.728

10. An electrophoretically cryptic alcohol dehydrogenase variant in Drosophila melanogaster. I. Activity ratios, thermostability, genetic localization and comparison with two other thermostable variants.

Authors: J B Gibson; G K Chambers; A V Wilks; J G Oakeshott
Journal: Aust J Biol Sci Date: 1980-08

3 in total