Yoav Chemla1,2, Oshra Betzer2,3, Amos Markus1, Nairouz Farah1, Menachem Motiei2,3, Rachela Popovtzer2,3, Yossi Mandel1,2. 1. Faculty of Life Sciences, School of Optometry & Vision Science, Bar-Ilan University, Ramat Gan 5290002, Israel. 2. Bar-Ilan Institute for Nanotechnology & Advanced Materials (BINA), Bar-Ilan University, Ramat Gan 5290002, Israel. 3. Faculty of Engineering, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Abstract
Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.
Aim: Longitudinal tracking of transplanted cells in clinical and experimental setups is crucial for evaluating the efficiency of retinal cell replacement therapies. Materials & methods: Gold nanoparticle-labeled photoreceptor precursors were transplanted in the vitreous and subretinal space of rats and were longitudinally tracked for over a month using optical coherence tomography, computed tomography and fluorescence fundus imaging. Results: This multimodal imaging approach enabled high-resolution long-term tracking and estimation of cell survival in the retina and vitreous, while displaying no toxic effects on the cells or the retina. Conclusion: These observations highlight the applicability of using gold nanoparticle for retinal cell tracking in existing experimental settings and its translational potential for providing more efficient retinal cell therapy in humans.
Authors: Jessica C Hsu; Lenitza M Nieves; Oshra Betzer; Tamar Sadan; Peter B Noël; Rachela Popovtzer; David P Cormode Journal: Wiley Interdiscip Rev Nanomed Nanobiotechnol Date: 2020-05-22
Authors: Jennifer Oberländer; Rafael Ayerbe; Joan Cabellos; Richard da Costa Marques; Bin Li; Nazende Günday-Türeli; Akif Emre Türeli; Racheli Ofir; Eliran Ish Shalom; Volker Mailänder Journal: Cells Date: 2022-07-28 Impact factor: 7.666