BACKGROUND: The mosquito, Aedes aegypti (Diptera: Culicidae), is a vector of dengue fever, zika, chikungunya, and yellow fever viruses, and in many areas possesses significant levels of resistance to pyrethroids. Behavioral performance was assessed in 15, 30, and 60 min exposures in a high throughput vapor phase spatial repellency assay to three contact repellent standards: N,N-diethyl-3-methylbenzamide (DEET), ethyl 3-[acetyl(butyl)amino] propanoate (IR3535), and 2-undecanone, as well as pyrethrum extract, transfluthrin, and metofluthrin in susceptible (Orlando) and a pyrethroid-resistant Puerto Rico strain of Aedes aegypti. Additionally, electroantennographic studies were used to investigate the antennal sensitivities to these compounds in both strains. RESULTS: Resistance was found to all tested insect repellents in the Puerto Rico strain of Ae. aegypti. Resistance ratios at the different time points were about 2 for DEET, 3 for 2-undecanone, and 12 for IR3535. Resistance was also observed to pyrethrum extract (∼9-fold), transfluthrin (∼5-fold), and metofluthrin (∼48-fold) in repellent behavioral response. Electrophysiological analysis found decreased antennal sensitivity to all repellents tested, consistent with their behavioral effects. CONCLUSION: The reduced sensitivity to these repellents may represent a fitness cost arising from the kdr mutation present in Puerto Rico Aedes aegypti. This work highlights the need for understanding collateral effects from the evolution of pesticide resistance in mosquitoes, and the importance of finding alternative strategies to control resistance development.
BACKGROUND: The mosquito, Aedes aegypti (Diptera: Culicidae), is a vector of dengue fever, zika, chikungunya, and yellow fever viruses, and in many areas possesses significant levels of resistance to pyrethroids. Behavioral performance was assessed in 15, 30, and 60 min exposures in a high throughput vapor phase spatial repellency assay to three contact repellent standards: N,N-diethyl-3-methylbenzamide (DEET), ethyl 3-[acetyl(butyl)amino] propanoate (IR3535), and 2-undecanone, as well as pyrethrum extract, transfluthrin, and metofluthrin in susceptible (Orlando) and a pyrethroid-resistant Puerto Rico strain of Aedes aegypti. Additionally, electroantennographic studies were used to investigate the antennal sensitivities to these compounds in both strains. RESULTS: Resistance was found to all tested insect repellents in the Puerto Rico strain of Ae. aegypti. Resistance ratios at the different time points were about 2 for DEET, 3 for 2-undecanone, and 12 for IR3535. Resistance was also observed to pyrethrum extract (∼9-fold), transfluthrin (∼5-fold), and metofluthrin (∼48-fold) in repellent behavioral response. Electrophysiological analysis found decreased antennal sensitivity to all repellents tested, consistent with their behavioral effects. CONCLUSION: The reduced sensitivity to these repellents may represent a fitness cost arising from the kdr mutation present in Puerto Rico Aedes aegypti. This work highlights the need for understanding collateral effects from the evolution of pesticide resistance in mosquitoes, and the importance of finding alternative strategies to control resistance development.
Authors: Wilson Valbon; Felipe Andreazza; Eugenio E Oliveira; Feng Liu; Bo Feng; Maura Hall; James Klimavicz; Joel R Coats; Ke Dong Journal: Pest Manag Sci Date: 2021-10-27 Impact factor: 4.845
Authors: Lisa M Rigby; Brian J Johnson; Christopher L Peatey; Nigel W Beebe; Gregor J Devine Journal: Pest Manag Sci Date: 2021-05-06 Impact factor: 4.845
Authors: Morgana do Nascimento Xavier; Marina Praxedes Rodrigues; Danielle Cristina Tenório Varjal de Melo; Eloína Maria de Mendonça Santos; Rosângela Maria Rodrigues Barbosa; Cláudia Maria Fontes de Oliveira Journal: Rev Inst Med Trop Sao Paulo Date: 2020-11-27 Impact factor: 1.846
Authors: Lisa M Rigby; Brian J Johnson; Gordana Rašić; Christopher L Peatey; Leon E Hugo; Nigel W Beebe; Gunter F Hartel; Gregor J Devine Journal: PLoS Negl Trop Dis Date: 2021-02-05
Authors: Feng Liu; Qiang Wang; Peng Xu; Felipe Andreazza; Wilson R Valbon; Elizabeth Bandason; Mengli Chen; Ru Yan; Bo Feng; Leticia B Smith; Jeffrey G Scott; Genki Takamatsu; Makoto Ihara; Kazuhiko Matsuda; James Klimavicz; Joel Coats; Eugenio E Oliveira; Yuzhe Du; Ke Dong Journal: Nat Commun Date: 2021-05-05 Impact factor: 14.919
Authors: Ingeborg H Cuba; Gary R Richoux; Edmund J Norris; Ulrich R Bernier; Kenneth J Linthicum; Jeffrey R Bloomquist Journal: Curr Res Parasitol Vector Borne Dis Date: 2021-11-18