| Literature DB >> 3133450 |
Abstract
Viral genomic RNA was purified from BHK-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsRNA genome segments were isolated by polyacrylamide gel electrophoresis. These genome segments were translated in vitro using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. This allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified proteins not readily detectable in lysates of virus-infected cells.Entities:
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Year: 1988 PMID: 3133450 DOI: 10.1099/0022-1317-69-6-1255
Source DB: PubMed Journal: J Gen Virol ISSN: 0022-1317 Impact factor: 3.891