Literature DB >> 3133450

Protein coding assignment for the genome of epizootic haemorrhagic disease virus.

J O Mecham1, V C Dean.   

Abstract

Viral genomic RNA was purified from BHK-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsRNA genome segments were isolated by polyacrylamide gel electrophoresis. These genome segments were translated in vitro using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. This allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified proteins not readily detectable in lysates of virus-infected cells.

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Year:  1988        PMID: 3133450     DOI: 10.1099/0022-1317-69-6-1255

Source DB:  PubMed          Journal:  J Gen Virol        ISSN: 0022-1317            Impact factor:   3.891


  17 in total

1.  Bluetongue virus VP6 protein binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates.

Authors:  N Stäuber; J Martinez-Costas; G Sutton; K Monastyrskaya; P Roy
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

2.  Epizootic hemorrhagic disease: analysis of tissues by amplification and in situ hybridization reveals widespread orbivirus infection at low copy numbers.

Authors:  S J Brodie; K D Bardsley; K Diem; J O Mecham; S E Norelius; W C Wilson
Journal:  J Virol       Date:  1998-05       Impact factor: 5.103

3.  Site-specific mutations in the NS2 protein of epizootic haemorrhagic disease virus markedly affect the formation of cytoplasmic inclusion bodies.

Authors:  J Theron; H Huismans; L H Nel
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

4.  Molecular evolution of epizootic hemorrhagic disease viruses in North America based on historical isolates using motif fingerprints.

Authors:  W C Wilson; M G Ruder; D Jasperson; T P L Smith; P Naraghi-Arani; R Lenhoff; D E Stallknecht; W A Valdivia-Granda; D Sheoran
Journal:  Virus Genes       Date:  2016-04-23       Impact factor: 2.332

5.  A comparison of different cloned genome segments of epizootic haemorrhagic disease virus as serogroup-specific probes.

Authors:  L H Nel; H Huismans
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

6.  A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1.

Authors:  M J Jensen; W C Wilson
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

7.  PCR detection of North American and Central African isolates of epizootic hemorrhagic disease virus (EHDV) based on genome segment 10 of EHDV serotype 1.

Authors:  I E Aradaib; W C Wilson; C E Schore; M E Mohammed; T D Yilma; J S Cullor; B I Osburn
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

8.  The evolution of two homologues of the core protein VP6 of epizootic haemorrhagic disease virus (EHDV), which correspond to the geographical origin of the virus.

Authors:  S J Anthony; K E Darpel; S Maan; G Sutton; H Attoui; P P C Mertens
Journal:  Virus Genes       Date:  2009-10-15       Impact factor: 2.332

9.  RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.

Authors:  Narender S Maan; Sushila Maan; Kyriaki Nomikou; Donna J Johnson; Mehdi El Harrak; Hafsa Madani; Hagai Yadin; Serife Incoglu; Kadir Yesilbag; Andrew B Allison; David E Stallknecht; Carrie Batten; Simon J Anthony; Peter P C Mertens
Journal:  PLoS One       Date:  2010-09-17       Impact factor: 3.240

10.  Development of PCR-based tests for the identification of North American isolates of epizootic haemorrhagic disease virus.

Authors:  M J Harding; I Prud'homme; J Rola; G C Dulac
Journal:  Can J Vet Res       Date:  1996-01       Impact factor: 1.310

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