Iman Azari1, Soudeh Ghafouri-Fard1, Mir Davood Omrani2, Shahram Arsang-Jang3, Dor Mohammad Kordi Tamandani4, Mehrnaz Saroone Rigi5, Sara Rafiee4, Farkhondeh Pouresmaeili1,6, Mohammad Taheri2. 1. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Clinical Research Development Center (CRDU), Qom University of Medical Sciences, Qom, Iran. 4. Department of Biology, University of Sistan and Baluchistan, Zahedan- Iran. 5. Shafa Surgery Center, Zahedan, Sistan and Baluchistan, Iran. 6. nfertility and Reproductive Health Research Center (IRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Intrauterine growth restriction (IUGR), a pathologic diminution of the rate of fetal growth, has been associated with alterations in expression of several genes. However, the role of long non-coding RNAs (lncRNAs) in its pathogenesis has not been studied. METHODS: In this study we evaluated the expression of four lncRNAs namely, nuclear paraspeckle assembly transcript (NEAT1), taurine up-regulated 1 (TUG1), p21-associated ncRNA DNA damage-activated (PANDA), and metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) in placenta samples obtained from IUGR and normal pregnancies to determine their possible contributions in the pathogenesis of IUGR. RESULTS: We found no significant differences in expression levels between cases and controls. We also found no correlation between expression and clinical data of study participants; however, we found significant correlations between expression levels of all the assessed lncRNAs in both cases and controls. CONCLUSION: These results imply the existence of a possible shared regulatory mechanism for the expression of these transcripts in placenta. Future studies are needed to perform such evaluations in larger sample sizes or in animal models in earlier stages of pregnancy.
BACKGROUND: Intrauterine growth restriction (IUGR), a pathologic diminution of the rate of fetal growth, has been associated with alterations in expression of several genes. However, the role of long non-coding RNAs (lncRNAs) in its pathogenesis has not been studied. METHODS: In this study we evaluated the expression of four lncRNAs namely, nuclear paraspeckle assembly transcript (NEAT1), taurine up-regulated 1 (TUG1), p21-associated ncRNA DNA damage-activated (PANDA), and metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) in placenta samples obtained from IUGR and normal pregnancies to determine their possible contributions in the pathogenesis of IUGR. RESULTS: We found no significant differences in expression levels between cases and controls. We also found no correlation between expression and clinical data of study participants; however, we found significant correlations between expression levels of all the assessed lncRNAs in both cases and controls. CONCLUSION: These results imply the existence of a possible shared regulatory mechanism for the expression of these transcripts in placenta. Future studies are needed to perform such evaluations in larger sample sizes or in animal models in earlier stages of pregnancy.
Authors: Attila Rab; Imre Szentpéteri; László Kornya; Balázs Börzsönyi; Csaba Demendi; József Gábor Joó Journal: Eur J Obstet Gynecol Reprod Biol Date: 2013-06-27 Impact factor: 2.435
Authors: Aleksandra Lipka; Jan Pawel Jastrzebski; Lukasz Paukszto; Karol Gustaw Makowczenko; Elzbieta Lopienska-Biernat; Marek Gowkielewicz; Ewa Lepiarczyk; Marta Wiszpolska; Mariusz Krzysztof Majewski; Marta Majewska Journal: Cells Date: 2021-04-16 Impact factor: 6.600