Development of a two-photon fluorescent probe to monitor the changes of viscosity in living cells, zebra fish and mice.

The detection of viscosity is of great significance for medical research. Herein, we have developed a two-photon fluorescent probe CB-V for monitoring micro-viscosity changes. The fluorescence emission intensity of CB-V increased 9.6-fold from methanol to glycerol exhibiting an excellent fluorescence response. With excellent properties of CB-V, monitoring the viscosity variations has been achieved not only in living cells but also in zebra fish and mice.