Grid columnar flat panel photobioreactor with immobilized photosynthetic bacteria for continuous photofermentative hydrogen production.

A biophotoreactor with a transparent glass flat panel with polymethyl methacrylate (PMMA) grid columnar for enhanced biofilm growth with Rhodopseudomonas palustris GCA009 was developed and tested at 590 nm incident light. Continuous photofermentative hydrogen production from glucose was tested using this novel reactor. At light intensity of 210 W/m2, feed substrate concentration of 56.0 mmol/L, and crossflow velocity of 1.68 × 10-6 m/s, a maximum hydrogen production rate of 32.6 mmol/L-d (3.56 mmol/m2-h), hydrogen yield of 1.15 mol H2/mol glucose and light conversion efficiency of 5.34% can be achieved. Since the revised grid columnar effectively enlarged the surface area of reactor and enhanced cell attachment, the present reactor design led to higher hydrogen production rates than literature works.