| Literature DB >> 31326021 |
Sharon G Wolf1, Michael Elbaum2.
Abstract
Electron cryo-tomography using the scanning transmission modality (STEM) enables 3D reconstruction of unstained, vitrified specimens as thick as 1μm or more. Contrast is related to mass/thickness and atomic number, providing quantifiable chemical characterization and mass mapping of intact prokaryotic and eukaryotic cells. Energy dispersive X-ray spectroscopy by STEM provides a simple, on-the-spot chemical identification of the elemental composition in sub-cellular organic bodies or mineral deposits. This chapter provides basic background and practical information for performing cryo-STEM tomography on vitrified biological cells.Keywords: Amorphous calcium phosphate; CSTET; EDS; EDX; Electron tomography; Energy-dispersive X-ray spectroscopy; Ferritin; Metalloprotein; Mitochondrial deposits; Polyphosphate bodies; Scanning probe microscopy; Scanning transmission electron microscopy (STEM); Z contrast; cryo-EM; cryo-microscopy
Mesh：
Year: 2019 PMID： 31326021 DOI： 10.1016/bs.mcb.2019.04.001
Source DB: PubMed Journal: Methods Cell Biol ISSN： 0091-679X Impact factor: 1.441