Jeremiah Seni1, Stephen E Mshana1, Felician Msigwa1, Shabani Iddi2, Humphrey Mazigo3, Julian Parkhill4, Mark A Holmes5, Gavin K Paterson6. 1. Department of Microbiology/Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Science, Mwanza, Tanzania. 2. Department of Physiology, Weill Bugando School of Medicine, Catholic University of Health and Allied Science, Mwanza, Tanzania. 3. Department of Parasitology and Entomology, Weill Bugando School of Medicine, Catholic University of Health and Allied Science, Mwanza, Tanzania. 4. Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, UK. 5. Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. 6. Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, UK. Electronic address: gavin.paterson@ed.ac.uk.
Abstract
OBJECTIVES: Coagulase-negative staphylococci such as Staphylococcus cohnii are opportunistic pathogens of humans and animals. A multidrug-resistant isolate of S. cohnii subsp. urealyticus (073AN) was isolated from the nasal cavity of a healthy goat in Tanzania. This study produced and analysed a draft genome sequence of strain 073AN to investigate the genetic basis for antimicrobial resistance in this isolate. METHODS: Strain 073AN was sequenced using HiSeq 2000 technology, sequencing reads were assembled using Velvet, and the genome was annotated using Prokka. RESULTS: The draft genome of strain 073AN is 2677652bp in size with a GC content of 32.5%. The isolate was resistant to several classes of antimicrobials, which correlated with the presence of known antimicrobial resistance genes. Of particular note, the draft genome sequence of strain 073AN represents the first report of fosD in S. cohnii and the first descriptions of fosD and fusF in Africa. CONCLUSION: The draft genome sequence of S. cohnii subsp. urealyticus 073AN released here provides an insight into the antimicrobial resistance determinants found in this species and in Tanzania and offers a valuable resource for further studies on staphylococcal genomics and antimicrobial resistance.
OBJECTIVES: Coagulase-negative staphylococci such as Staphylococcus cohnii are opportunistic pathogens of humans and animals. A multidrug-resistant isolate of S. cohnii subsp. urealyticus (073AN) was isolated from the nasal cavity of a healthy goat in Tanzania. This study produced and analysed a draft genome sequence of strain 073AN to investigate the genetic basis for antimicrobial resistance in this isolate. METHODS: Strain 073AN was sequenced using HiSeq 2000 technology, sequencing reads were assembled using Velvet, and the genome was annotated using Prokka. RESULTS: The draft genome of strain 073AN is 2677652bp in size with a GC content of 32.5%. The isolate was resistant to several classes of antimicrobials, which correlated with the presence of known antimicrobial resistance genes. Of particular note, the draft genome sequence of strain 073AN represents the first report of fosD in S. cohnii and the first descriptions of fosD and fusF in Africa. CONCLUSION: The draft genome sequence of S. cohnii subsp. urealyticus 073AN released here provides an insight into the antimicrobial resistance determinants found in this species and in Tanzania and offers a valuable resource for further studies on staphylococcal genomics and antimicrobial resistance.
Authors: Tobias Lienen; Arne Schnitt; Jens Andre Hammerl; Stephen F Marino; Sven Maurischat; Bernd-Alois Tenhagen Journal: Sci Rep Date: 2021-03-16 Impact factor: 4.379