Ishita Bhavsar1, Craig S Miller2, Jeffrey L Ebersole3, Dolphus R Dawson1, Katherine L Thompson4, Mohanad Al-Sabbagh1. 1. Department of Oral Health Practice, Division of Periodontology, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA. 2. Department of Oral Health Practice, Division of Oral Diagnosis, Oral Medicine and Oral Radiology, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA. 3. Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, Kentucky, USA. 4. Department of Statistics, College of Arts & Sciences, University of Kentucky, Lexington, Kentucky, USA.
Abstract
OBJECTIVE: To investigate biological markers of peri-implantitis (PIP) in crevicular fluid before and after surgical and antimicrobial therapy. MATERIAL AND METHODS: Forty-eight participants (24 healthy implants and 24 PIP) were clinically evaluated, and peri-implant crevicular fluid (PICF) samples were collected at baseline for both groups, and at 3-months after surgical and antimicrobial treatment (ie, n = 21 PIP completers). Samples were analyzed for interleukin-1β (IL-1β), matrix metalloproteinase-8 (MMP-8), and macrophage inflammatory protein-1α (MIP-1α) using immunoassay and the results compared between groups. RESULTS: Peri-implantitis sites at baseline demonstrated significantly higher mean periodontal probing depths, percentage bleeding on probing (P ≤ 0.001), and mean IL-1β concentration in PICF compared to healthy implant sites (17.9 vs 1.7 pg/μL; P = 0.02). Three months after treatment, periodontal probing depths, bleeding on probing, suppuration (P < 0.05), and the mean concentration of MMP-8 decreased significantly compared with baseline (12.1 vs 6.7 ng/μL, P = 0.04). MIP-1α concentrations showed no differences between the groups. CONCLUSION: Elevated concentrations of IL-1β in PICF were consistent with PIP. A decrease in MMP-8 concentration in PICF at three months after treatment is consistent with a healing biological response.
OBJECTIVE: To investigate biological markers of peri-implantitis (PIP) in crevicular fluid before and after surgical and antimicrobial therapy. MATERIAL AND METHODS: Forty-eight participants (24 healthy implants and 24 PIP) were clinically evaluated, and peri-implant crevicular fluid (PICF) samples were collected at baseline for both groups, and at 3-months after surgical and antimicrobial treatment (ie, n = 21 PIP completers). Samples were analyzed for interleukin-1β (IL-1β), matrix metalloproteinase-8 (MMP-8), and macrophage inflammatory protein-1α (MIP-1α) using immunoassay and the results compared between groups. RESULTS:Peri-implantitis sites at baseline demonstrated significantly higher mean periodontal probing depths, percentage bleeding on probing (P ≤ 0.001), and mean IL-1β concentration in PICF compared to healthy implant sites (17.9 vs 1.7 pg/μL; P = 0.02). Three months after treatment, periodontal probing depths, bleeding on probing, suppuration (P < 0.05), and the mean concentration of MMP-8 decreased significantly compared with baseline (12.1 vs 6.7 ng/μL, P = 0.04). MIP-1α concentrations showed no differences between the groups. CONCLUSION: Elevated concentrations of IL-1β in PICF were consistent with PIP. A decrease in MMP-8 concentration in PICF at three months after treatment is consistent with a healing biological response.
Authors: Glaucia Schuindt Teixeira Neves; Gayathiri Elangovan; Mayla Kezy Silva Teixeira; João Martins de Mello-Neto; Santosh Kumar Tadakamadla; Eduardo José Veras Lourenço; Daniel Moraes Telles; Carlos Marcelo Figueredo Journal: Int J Environ Res Public Health Date: 2022-03-18 Impact factor: 4.614