Joseph M Kulinski1, Robin Eisch1, Michael L Young2, Shakuntala Rampertaap3, Jennifer Stoddard3, Joseph Monsale3, Kimberly Romito3, Jonathan J Lyons1, Sergio D Rosenzweig3, Dean D Metcalfe1, Hirsh D Komarow4. 1. Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md. 2. Clinical Research Directorate, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, Md. 3. Immunology Service, Department of Laboratory Medicine, National Institutes of Health Clinical Center, National Institutes of Health, Bethesda, Md. 4. Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md. Electronic address: komarowh@niaid.nih.gov.
Abstract
BACKGROUND: Mastocytosis is a clonal mast cell disorder associated with elevated mast cell mediators, which themselves have been reported to affect lymphocyte function. However, the impact of an expanded mast cell compartment on lymphocyte subpopulations, and their correlation with clinical phenotypes in patients with indolent systemic mastocytosis (ISM), has not been explored. OBJECTIVE: To examine the immunophenotype of circulating lymphocytes in patients with ISM compared with healthy adult controls and examine relationships with aspects of clinical disease. METHODS: We examined lymphocyte subsets in 20 adult patients with ISM and 40 healthy adult volunteers by multiparameter flow cytometry. Results were correlated with clinical characteristics. RESULTS: Patients with ISM exhibited a significantly lower median frequency and absolute cell count of both circulating CD8+ T cells and natural killer cells accompanying a significantly increased ratio of CD4+/CD8+ T cells when compared with healthy volunteers. Stratification of our ISM patient cohort according to clinical manifestations revealed that CD19+CD21lowCD38low B cells were significantly higher in patients with a history of autoimmune disease and counts of terminally differentiated CD4+ T cells were significantly higher in patients with osteoporosis or osteopenia. CONCLUSIONS: Several circulating lymphocyte subpopulations in patients with ISM were significantly different when compared with healthy controls; in specific lymphocyte subsets, this lymphocyte skewing correlated with clinical observations including osteoporosis and autoimmune disease. These data suggest the need for further studies on abnormalities in lymphocyte subsets and the attendant clinical consequences in both mast cell proliferative and activation disorders. Published by Elsevier Inc.
BACKGROUND:Mastocytosis is a clonal mast cell disorder associated with elevated mast cell mediators, which themselves have been reported to affect lymphocyte function. However, the impact of an expanded mast cell compartment on lymphocyte subpopulations, and their correlation with clinical phenotypes in patients with indolent systemic mastocytosis (ISM), has not been explored. OBJECTIVE: To examine the immunophenotype of circulating lymphocytes in patients with ISM compared with healthy adult controls and examine relationships with aspects of clinical disease. METHODS: We examined lymphocyte subsets in 20 adult patients with ISM and 40 healthy adult volunteers by multiparameter flow cytometry. Results were correlated with clinical characteristics. RESULTS:Patients with ISM exhibited a significantly lower median frequency and absolute cell count of both circulating CD8+ T cells and natural killer cells accompanying a significantly increased ratio of CD4+/CD8+ T cells when compared with healthy volunteers. Stratification of our ISM patient cohort according to clinical manifestations revealed that CD19+CD21lowCD38low B cells were significantly higher in patients with a history of autoimmune disease and counts of terminally differentiated CD4+ T cells were significantly higher in patients with osteoporosis or osteopenia. CONCLUSIONS: Several circulating lymphocyte subpopulations in patients with ISM were significantly different when compared with healthy controls; in specific lymphocyte subsets, this lymphocyte skewing correlated with clinical observations including osteoporosis and autoimmune disease. These data suggest the need for further studies on abnormalities in lymphocyte subsets and the attendant clinical consequences in both mast cell proliferative and activation disorders. Published by Elsevier Inc.
Entities:
Keywords:
Flow cytometry; Lymphocyte; Mast cell activation; Mast cells; Mastocytosis; NK cell; T cell; Tryptase
Authors: Alba Pérez-Pons; María Jara-Acevedo; Ana Henriques; Paula Navarro-Navarro; Andrés C García-Montero; Iván Álvarez-Twose; Carlos E Pedreira; Laura Sánchez-Muñoz; Daniela Damasceno; Carolina Caldas; Javier I Muñoz-González; Almudena Matito; Juan Flores-Montero; Oscar González-López; Ignacio Criado; Andrea Mayado; Alberto Orfao Journal: Clin Transl Allergy Date: 2022-06-14 Impact factor: 5.657
Authors: Liza Konnikova; Tanya O Robinson; Anna H Owings; James F Shirley; Elisabeth Davis; Ying Tang; Sarah Wall; Jian Li; Mohammad H Hasan; Raad Z Gharaibeh; Lybil B Mendoza Alvarez; Lisa K Ryan; Andria Doty; Jack F Chovanec; Michael P O'Connell; Dianne E Grunes; William P Daley; Emeran Mayer; Lin Chang; Julia Liu; Scott B Snapper; Joshua D Milner; Sarah C Glover; Jonathan J Lyons Journal: J Allergy Clin Immunol Date: 2021-04-15 Impact factor: 14.290