Literature DB >> 3131436

Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells.

C Czerkinsky1, G Andersson, H P Ekre, L A Nilsson, L Klareskog, O Ouchterlony.   

Abstract

A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.

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Year:  1988        PMID： 3131436     DOI： 10.1016/0022-1759(88)90079-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  92 in total

1.  Identification of naturally processed and HLA-presented Epstein-Barr virus peptides recognized by CD4(+) or CD8(+) T lymphocytes from human blood.

Authors:  W Herr; E Ranieri; A Gambotto; L S Kierstead; A A Amoscato; L Gesualdo; W J Storkus
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

2.  Development of a novel in vitro assay (ALS assay) for evaluation of vaccine-induced antibody secretion from circulating mucosal lymphocytes.

Authors:  H S Chang; D A Sack
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

3.  Lysosomal cathepsin B plays an important role in antigen processing, while cathepsin D is involved in degradation of the invariant chain inovalbumin-immunized mice.

Authors:  T Zhang; Y Maekawa; J Hanba; T Dainichi; B F Nashed; H Hisaeda; T Sakai; T Asao; K Himeno; R A Good; N Katunuma
Journal:  Immunology       Date:  2000-05       Impact factor: 7.397

4.  Quantification of antigen-reactive T cells by a modified ELISPOT assay based on freshly isolated blood dendritic cells.

Authors:  M Schmitz; J Rohayem; R Paul; B Weigle; A Stein; E P Rieber
Journal:  J Clin Lab Anal       Date:  2002       Impact factor: 2.352

5.  Discrepancy between ELISPOT IFN-gamma secretion and binding of A2/peptide multimers to TCR reveals interclonal dissociation of CTL effector function from TCR-peptide/MHC complexes half-life.

Authors:  V Rubio-Godoy; V Dutoit; D Rimoldi; D Lienard; F Lejeune; D Speiser; P Guillaume; J C Cerottini; P Romero; D Valmori
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-21       Impact factor: 11.205

6.  Altering the cellular location of an antigen expressed by a DNA-based vaccine modulates the immune response.

Authors:  P J Lewis; L A Babiuk
Journal:  J Virol       Date:  1999-12       Impact factor: 5.103

7.  Modulation of immune responses and suppression of experimental autoimmune myasthenia gravis by surgical denervation of the spleen.

Authors:  M Bakhiet; L-Y Yu; V Ozenci; A Khan; F-D Shi
Journal:  Clin Exp Immunol       Date:  2006-05       Impact factor: 4.330

8.  Sampling tumor-draining lymph nodes for phenotypic and functional analysis of dendritic cells and T cells.

Authors:  Ronald J C L M Vuylsteke; Paul A M van Leeuwen; Sybren Meijer; Pepijn G J T B Wijnands; Markwin G Statius Muller; Dirk H Busch; Rik J Scheper; Tanja D de Gruijl
Journal:  Am J Pathol       Date:  2002-07       Impact factor: 4.307

9.  Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.

Authors:  Nan Deng; Tim R Mosmann
Journal:  Cytometry A       Date:  2015-04-28       Impact factor: 4.355

10.  T cell responses to orbital antigens in thyroid-associated ophthalmopathy.

Authors:  K Arnold; N Tandon; R S McIntosh; R Elisei; M Ludgate; A P Weetman
Journal:  Clin Exp Immunol       Date:  1994-05       Impact factor: 4.330
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