Metal-Organic Framework Loaded by Rhodamine B As a Novel Chemiluminescence System for the Paper-Based Analytical Devices and Its Application for Total Phenolic Content Determination in Food Samples.

Herein, a novel paper-based chemiluminescence (CL) device has been reported for the estimation of total phenolic content of food samples. The CL system implemented on the paper was based on a hydrogen peroxide (H2O2)-rhodamine b (RhoB)-cobalt metal organic framework (CoMOF) reaction. It was found that the reaction of H2O2 with RhoB molecules, loaded into the nanopores of CoMOF (R@CoMOF), can produce an intensive CL emission. The experiments on the paper indicated that in the presence of CoMOF, the CL emission was greatly increased. In addition to this strong catalyzing effect, application of CoMOF on the paper improved the stability of the CL system for several days. As a useful analytical application for the obtained paper-based CL device (PCD), it was examined for the detection of phenolic antioxidants. It was observed that the addition of 5 μL of phenolic compounds (PC) on the paper containing the CL reagents can remarkably decrease the CL intensity. This effect was applied to design a simple analytical assay for PC. After the optimization process, the best sensitivity was obtained for gallic acid, quercetin, catechin, kaempferol, and caffeic acid with detection limits of 0.98, 1.36, 1.48, 1.81, and 2.55 ng mL-1, respectively. The relative standard deviations (RSD%) were also less than 5%. This study is the first report on the practical application of PCD using a nanomaterial assisted CL reaction. It is simple, portable, and low-cost and consumes a very low amount of reagents and sample solution. The device was successfully applied in the investigation of total antioxidant capacity of molasses and honey samples.