A pure shift and spin echo based approach for high-resolution diffusion-ordered NMR spectroscopy.

Diffusion-ordered NMR spectroscopy (DOSY) can be used for separating mixture components according to their individual diffusion behaviors, thus offering a powerful tool for the analysis of compound mixtures. However, conventional DOSY experiments generally encounter the problem of limited resolution in the spectral domain, particularly for applications to complex mixtures that contains crowed resonances in 1D NMR. In addition, chemical exchange effects, bringing about spurious component signals, pose another limitation for interpreting DOSY measurements. Here, a general DOSY method is proposed based on pure shift extraction and spin echo evolution to obtain high-resolution 2D DOSY spectra, along with the suppression on effects of chemical exchange and J coupling. Both theoretical analyses and experimental results suggest that the proposed method is useful for high-resolution DOSY measurements on complex mixtures that contains crowded or even overlapped NMR resonances and exchanging spin systems.