Literature DB >> 3130294

Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268.

B Boizet1, D Villeval, P Slos, M Novel, G Novel, A Mercenier.   

Abstract

A 4.4-kb XhoI fragment of Streptococcus lactis L13 (Z268) lactose plasmid pUCL13, containing the beta-D-phosphogalactoside galactohydrolase (P-beta Gal; EC 3.2.1.85)-coding gene has been cloned in Escherichia coli. Further subcloning and deletion of this fragment allowed localization of the P-beta Gal-coding gene (pbg) on a minimal 1.8-kb segment. Expression of P-beta Gal activity was constitutive and was not regulated by glucose in E. coli. The presence of P-beta Gal activity was correlated with the production of a 56.5-kDa protein in E. coli minicells. The nucleotide sequence of the cloned gene was determined and potential promoter structural elements were identified.

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Year:  1988        PMID： 3130294     DOI： 10.1016/0378-1119(88)90563-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  In vitro expression of Lac-PTS and tagatose 1,6-bisphosphate aldolase genes from Lactococcus lactis subsp. cremoris plasmid pDI-21.

Authors:  P L Yu; R A Hodge; X P Li
Journal:  Appl Microbiol Biotechnol       Date:  1990-09       Impact factor: 4.813

2.  Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification.

Authors:  E I Tanskanen; D L Tulloch; A J Hillier; B E Davidson
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

3.  Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons.

Authors:  D L Tulloch; L R Finch; A J Hillier; B E Davidson
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

4.  Presence of lactose genes and insertion sequences in plasmids of minor species of the genus lactococcus.

Authors:  S Bounaix; A Benachour; G Novel
Journal:  Appl Environ Microbiol       Date:  1996-03       Impact factor: 4.792

5.  Nucleotide and derived amino acid sequence of the cyanogenic beta-glucosidase (linamarase) from white clover (Trifolium repens L.).

Authors:  E Oxtoby; M A Dunn; A Pancoro; M A Hughes
Journal:  Plant Mol Biol       Date:  1991-08       Impact factor: 4.076

6.  Nucleotide sequences of the arb genes, which control beta-glucoside utilization in Erwinia chrysanthemi: comparison with the Escherichia coli bgl operon and evidence for a new beta-glycohydrolase family including enzymes from eubacteria, archeabacteria, and humans.

Authors:  M el Hassouni; B Henrissat; M Chippaux; F Barras
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

7.  Two Lactococcus lactis genes, including lacX, cooperate to trigger an SOS response in a recA-negative background.

Authors:  X F Huang; D C Huang; G Novel; M Novel
Journal:  J Bacteriol       Date:  1995-01       Impact factor: 3.490

8.  Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli.

Authors:  R M Wright; M D Yablonsky; Z P Shalita; A K Goyal; D E Eveleigh
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

9.  Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides.

Authors:  J Thompson; C R Gentry-Weeks; N Y Nguyen; J E Folk; S A Robrish
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

10.  Lactose transport system of Streptococcus thermophilus: a hybrid protein with homology to the melibiose carrier and enzyme III of phosphoenolpyruvate-dependent phosphotransferase systems.

Authors:  B Poolman; T J Royer; S E Mainzer; B F Schmidt
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490
  10 in total

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