Inhibition of urease activity by humic acid extracted from sludge fermentation liquid.

This study achieved effective extraction of humic acid from sludge fermentation liquid, and the inhibition of urease activity by the extract were investigated in the urea decomposition. The addition of extract could remarkably inhibit urease activity and extend the releasing time of ammonia nitrogen. The interaction between the extract and urease took times, and the inhibition was irreversible. The results of fluorescence analysis revealed that the inhibition of urease activity was correlated to the amount of humic acid extracted. The mechanisms of inhibition were proposed that the functional groups of humic acid might interact with the thiol group of urease and formed a larger particle size of complex to inhibit the activity of urease. The extraction of humic acid from sludge fermentation liquid can not only recover the resource from the fermentation liquid, but also provide a potential urease inhibitor for the sustained-release effect of the soil organic nitrogen fertilizer.