Reut Aviv Mordechai1, Shay Steinberg1, Liat Apel-Sarid2, Ety Shaoul3, Simona Zisman Rozen3, Lubna Khoury3, Ohad Ronen4,5. 1. Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. 2. Department of Pathology, Galilee Medical Center, Nahariya, Israel. 3. Department of Hematology, Galilee Medical Center, Nahariya, Israel. 4. Head and Neck Surgery Unit, Department of Otolaryngology-Head and Neck Surgery, Galilee Medical Center, POB 21, 2210001, Nahariya, Israel. ohadr@gmc.gov.il. 5. Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. ohadr@gmc.gov.il.
Abstract
INTRODUCTION: The presence of high-risk HPV in non-malignant tonsil tissues from patients who underwent tonsillectomy in the Galilee area might explain the low incidence of HPV-related oropharyngeal malignancy in the country. The aim of this retrospective study was to study the prevalence of human papillomavirus (HPV) in non-malignant tonsillectomy specimens of adults in the Galilee area. MATERIALS AND METHODS: We conducted a retrospective analysis of all tonsil samples in our medical center. Tonsils from patients over 20 years of age who underwent tonsillectomy for the indication of recurrent tonsillitis and sleep apneas were eligible. Samples of formalin-fixed paraffin-embedded (FFPE) tonsillar tissue were tested for the presence of HPV DNA using polymerase chain reaction and by p16 immunohistochemistry. RESULTS: Of the 71 tonsil samples, age range 20-65, none were positive for HPV DNA. Fifty-two FFPE specimens of tonsil with HPV-positive cancer tested positive by the same method served as positive controls. CONCLUSIONS: HPV DNA is rare in non-malignant tonsil tissues of young adults and adults who underwent tonsillectomy in the Galilee area. Further research should be done in larger cohorts.
INTRODUCTION: The presence of high-risk HPV in non-malignant tonsil tissues from patients who underwent tonsillectomy in the Galilee area might explain the low incidence of HPV-related oropharyngeal malignancy in the country. The aim of this retrospective study was to study the prevalence of human papillomavirus (HPV) in non-malignant tonsillectomy specimens of adults in the Galilee area. MATERIALS AND METHODS: We conducted a retrospective analysis of all tonsil samples in our medical center. Tonsils from patients over 20 years of age who underwent tonsillectomy for the indication of recurrent tonsillitis and sleep apneas were eligible. Samples of formalin-fixed paraffin-embedded (FFPE) tonsillar tissue were tested for the presence of HPV DNA using polymerase chain reaction and by p16 immunohistochemistry. RESULTS: Of the 71 tonsil samples, age range 20-65, none were positive for HPV DNA. Fifty-two FFPE specimens of tonsil with HPV-positive cancer tested positive by the same method served as positive controls. CONCLUSIONS:HPV DNA is rare in non-malignant tonsil tissues of young adults and adults who underwent tonsillectomy in the Galilee area. Further research should be done in larger cohorts.
Entities:
Keywords:
Human papillomavirus; Prevalence; Tonsillectomy
Authors: Cara C Cockerill; Laura J Orvidas; Eric J Moore; Matthew J Binnicker; Brian J Duresko; Mark J Espy; Franklin R Cockerill; Nicole M Tombers; Bobbi S Pritt Journal: Diagn Microbiol Infect Dis Date: 2016-08-21 Impact factor: 2.803
Authors: Anil K Chaturvedi; Eric A Engels; Ruth M Pfeiffer; Brenda Y Hernandez; Weihong Xiao; Esther Kim; Bo Jiang; Marc T Goodman; Maria Sibug-Saber; Wendy Cozen; Lihua Liu; Charles F Lynch; Nicolas Wentzensen; Richard C Jordan; Sean Altekruse; William F Anderson; Philip S Rosenberg; Maura L Gillison Journal: J Clin Oncol Date: 2011-10-03 Impact factor: 44.544
Authors: Gypsyamber D'Souza; Aimee R Kreimer; Raphael Viscidi; Michael Pawlita; Carole Fakhry; Wayne M Koch; William H Westra; Maura L Gillison Journal: N Engl J Med Date: 2007-05-10 Impact factor: 91.245
Authors: Monika Wojtera; Josee Paradis; Murad Husein; Anthony C Nichols; John W Barrett; Marina I Salvadori; Julie E Strychowsky Journal: J Otolaryngol Head Neck Surg Date: 2018-01-30