Literature DB >> 31299562

Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2).

Ruth Olmer1, Julia Dahlmann2, Sylvia Merkert2, Sandra Baus2, Gudrun Göhring3, Ulrich Martin2.   

Abstract

NK homeobox 1 (NKX2.1; also known as thyroid transcription factor 1, TTF-1) encodes for a transcription factor involved in the development of thyroid, lung and brain. Here, we established a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (iPSCs) using TALEN technology. The reporter enables the optimization and monitoring of the differentiation of pluripotent stem cells (PSCs) into NKX2.1 expressing cells on a single cell level, as well as the enrichment of NKX2.1 positive cells.
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

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Year:  2019        PMID： 31299562     DOI： 10.1016/j.scr.2019.101492

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  3 in total

1.  Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation.

Authors:  Markus Grosch; Sebastian Ittermann; Ejona Rusha; Tobias Greisle; Chaido Ori; Dong-Jiunn Jeffery Truong; Adam C O'Neill; Anna Pertek; Gil Gregor Westmeyer; Micha Drukker
Journal:  BMC Biol       Date:  2020-04-22       Impact factor: 7.431

2.  An NKX2-1GFP and TP63tdTomato dual fluorescent reporter for the investigation of human lung basal cell biology.

Authors:  Kim Jee Goh; Ee Kim Tan; Hao Lu; Sudipto Roy; N Ray Dunn
Journal:  Sci Rep       Date:  2021-02-25       Impact factor: 4.379

3.  Fgf10 Signaling-Based Evidence for the Existence of an Embryonic Stage Distinct From the Pseudoglandular Stage During Mouse Lung Development.

Authors:  Sara Taghizadeh; Matthew R Jones; Ruth Olmer; Saskia Ulrich; Soula Danopoulos; Chengguo Shen; Chaolei Chen; Jochen Wilhelm; Ulrich Martin; Chengshui Chen; Denise Al Alam; Saverio Bellusci
Journal:  Front Cell Dev Biol       Date:  2020-10-22
  3 in total

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