Y Yu1, J-D Zhao, H Yang. 1. Perinatal Care Division, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, P.R. China. yanghui_huaian@126.com.
Abstract
OBJECTIVE: Many studies have demonstrated that the abnormal microRNAs (miRNAs) expression plays crucial roles in the development of human cancers including cervical cancer (CC). However, the expression and the underlying mechanism of miR-299-3p in CC remain unclear. MATERIALS AND METHODS: In this study, the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect miR-299-3p expression level in CC cell lines. The cell proliferation assay, colony formation assay, and transwell invasion assay were conducted to investigate the biological functions of miR-299-3p. Followingly, the Luciferase activity reporter and the Western blot assays were conducted to validate the transcription factor 4 (TCF4) as a direct target of miR-299-3p. RESULTS: MiR-299-3p expression level was reduced in CC cell lines compared with the normal cell line. The overexpression of miR-299-3p inhibits CC cell growth and invasion. Furthermore, TCF4 was validated as a direct target of miR-299-3p. In addition, TCF4 overexpression reversed the inhibitory effects of miR-299-3p on CC cell behaviors. CONCLUSIONS: Taken together, our results illustrated that miR-299-3p acts as a tumor suppressor to inhibit CC cell behaviors by targeting TCF4.
OBJECTIVE: Many studies have demonstrated that the abnormal microRNAs (miRNAs) expression plays crucial roles in the development of humancancers including cervical cancer (CC). However, the expression and the underlying mechanism of miR-299-3p in CC remain unclear. MATERIALS AND METHODS: In this study, the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect miR-299-3p expression level in CC cell lines. The cell proliferation assay, colony formation assay, and transwell invasion assay were conducted to investigate the biological functions of miR-299-3p. Followingly, the Luciferase activity reporter and the Western blot assays were conducted to validate the transcription factor 4 (TCF4) as a direct target of miR-299-3p. RESULTS:MiR-299-3p expression level was reduced in CC cell lines compared with the normal cell line. The overexpression of miR-299-3p inhibits CC cell growth and invasion. Furthermore, TCF4 was validated as a direct target of miR-299-3p. In addition, TCF4 overexpression reversed the inhibitory effects of miR-299-3p on CC cell behaviors. CONCLUSIONS: Taken together, our results illustrated that miR-299-3p acts as a tumor suppressor to inhibit CC cell behaviors by targeting TCF4.