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Literature DB >> 3129345

Cytoplasmic membrane fluidity measurements on intact living cells of Bacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene.

Abstract

A method for determination of membrane fluidity (microviscosity) in Bacillus subtilis cytoplasmic membrane under in vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbance A as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).

Entities: Chemical Species

Mesh：

Substances：
Diphenylhexatriene

Year: 1988 PMID： 3129345 DOI： 10.1007/bf02928006

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

17 in total

Review 1. Fluidity parameters of lipid regions determined by fluorescence polarization.

Authors: M Shinitzky; Y Barenholz
Journal: Biochim Biophys Acta Date: 1978-12-15

2. Difference in microviscosity induced by different cholesterol levels in the surface membrane lipid layer of normal lymphocytes and malignant lymphoma cells.

Authors: M Shinitzky; M Inbar
Journal: J Mol Biol Date: 1974-01-05 Impact factor: 5.469

3. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.

Authors: A G Marr; J L Ingraham
Journal: J Bacteriol Date: 1962-12 Impact factor: 3.490

4. Relation between growth temperature of E. coli and phase transition temperatures of its cytoplasmic and outer membranes.

Authors: H Nakayama; T Mitsui; M Nishihara; M Kito
Journal: Biochim Biophys Acta Date: 1980-09-02

5. Fluorescence anisotropy measurements under oxygen quenching conditions as a method to quantify the depolarizing rotations of fluorophores. Application to diphenylhexatriene in isotropic solvents and in lipid bilayers.

Authors: J R Lakowicz; F G Prendergast; D Hogen
Journal: Biochemistry Date: 1979-02-06 Impact factor: 3.162

6. Use of fluorescence polarization to monitor intracellular membrane changes during temperature acclimation. Correlation with lipid compositional and ultrastructural changes.

Authors: C E Martin; G A Thompson
Journal: Biochemistry Date: 1978-08-22 Impact factor: 3.162

7. Microviscosity parameters and protein mobility in biological membranes.

Authors: M Shinitzky; M Inbar
Journal: Biochim Biophys Acta Date: 1976-04-16

8. Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdiphenylhexatriene.

Authors: J G Kuhry; G Duportail; C Bronner; G Laustriat
Journal: Biochim Biophys Acta Date: 1985-04-22

9. Resolution of plasma membrane lipid fluidity in intact cells labelled with diphenylhexatriene.

Authors: D Grunberger; R Haimovitz; M Shinitzky
Journal: Biochim Biophys Acta Date: 1982-06-28

10. Changes in membrane microviscosity associated with phagocytosis: effects of colchicine.

Authors: R D Berlin; J P Fera
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

5 in total

1. Rotational relaxation rate of 1,6-diphenyl-1,3,5-hexatriene in cytoplasmic membranes of Bacillus subtilis. A new model of heterogeneous rotations.

Authors: I Konopásek; J Svobodová; D D Toptygin; P Svoboda
Journal: Folia Microbiol (Praha) Date: 1990 Impact factor: 2.099