Literature DB >> 3129311

A mutant LexA repressor harboring a cleavage motif cysteine-glycine remains inducible.

M Granger-Schnarr1, P Oertel, M Schnarr.   

Abstract

Using site-directed mutagenesis of the lexA gene we have changed the amino acid Ala-84 of the LexA repressor for a cysteine. The reason for this change was the striking homology between LexA and UmuD and the comparable size of the two amino acid side chains. Using an in vivo repression/induction assay it is shown that the LexA-Cys-84 mutant remains inducible by mitomycin C and UV irradiation essentially in the same way as the wild-type repressor.

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Year:  1988        PMID： 3129311     DOI： 10.1016/0014-5793(88)80866-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  2 in total

1.  Genetic analysis of the LexA repressor: isolation and characterization of LexA(Def) mutant proteins.

Authors:  P Oertel-Buchheit; R M Lamerichs; M Schnarr; M Granger-Schnarr
Journal:  Mol Gen Genet       Date:  1990-08

2.  Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response.

Authors:  Luciane S Fonseca; Josefa B da Silva; Juliana S Milanez; Claudia B Monteiro-Vitorello; Leonardo Momo; Zenaide M de Morais; Silvio A Vasconcellos; Marilis V Marques; Paulo L Ho; Renata M A da Costa
Journal:  PLoS One       Date:  2013-10-03       Impact factor: 3.240
  2 in total

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