C Bichara1, B Berby1, A Rives1, F Jumeau1, M Letailleur2, V Setif1, L Sibert3, C Rondanino1, Nathalie Rives4. 1. Biology of Reproduction, CECOS Laboratory, Normandie Univ., UNIROUEN, EA 4308 "Gametogenesis and Gamete Quality", Rouen University Hospital, F 76000, Rouen, France. 2. ART Center, Normandie Univ., UNIROUEN, Rouen University Hospital, F 76000, Rouen, France. 3. Department of Urology-Andrology, Normandie Univ., UNIROUEN, EA 4308 "Gametogenesis and Gamete Quality", Rouen University Hospital, F 76000, Rouen, France. 4. Biology of Reproduction, CECOS Laboratory, Normandie Univ., UNIROUEN, EA 4308 "Gametogenesis and Gamete Quality", Rouen University Hospital, F 76000, Rouen, France. nathalie.rives@chu-rouen.fr.
Abstract
PURPOSE: The impact of sperm DNA damage on intracytoplasmic sperm injection (ICSI) outcomes remains controversial. The purpose of the study was to evaluate the prognostic value of several types of sperm nuclear damage on ICSI clinical pregnancy. METHODS: Our retrospective study included a total of 132 couples who consulted for male or mixed-factor infertility that benefited from ICSI cycles from January 2006 to December 2015. All infertile males presented at least one conventional semen parameter alteration. Sperm nuclear damage was assessed using the Motile Sperm Organelle Morphological Examination for sperm head relative vacuolar area (RVA), aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and fluorescence in situ hybridization for aneuploidy. RESULTS: Infertile males who achieved pregnancy after ICSI had fewer chromatin condensation defects than did males who did not achieve any pregnancy (15.8 ± 12.0% vs. 11.4 ± 7.9%, respectively, P = 0.0242), which remained significant in multivariate regression analysis (RR = 0.40 [0.18 to 0.86], P = 0.02). RVA, DNA fragmentation, and aneuploidy were not predictive factors of ICSI outcomes. The pregnancy rate was significantly decreased by number of progressive motile spermatozoa with normal morphology after migration (P = 0.04). In female partners, 17β estradiol of less than 2000 pg/mL on the day of ovulation induction significantly reduced the occurrence of clinical pregnancy (P = 0.04). CONCLUSION: Sperm chromatin condensation defects were more frequently observed in couples with ICSI failure and should be considered a negative predictive factor for the occurrence of clinical pregnancy.
PURPOSE: The impact of sperm DNA damage on intracytoplasmic sperm injection (ICSI) outcomes remains controversial. The purpose of the study was to evaluate the prognostic value of several types of sperm nuclear damage on ICSI clinical pregnancy. METHODS: Our retrospective study included a total of 132 couples who consulted for male or mixed-factor infertility that benefited from ICSI cycles from January 2006 to December 2015. All infertile males presented at least one conventional semen parameter alteration. Sperm nuclear damage was assessed using the Motile Sperm Organelle Morphological Examination for sperm head relative vacuolar area (RVA), aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and fluorescence in situ hybridization for aneuploidy. RESULTS: Infertile males who achieved pregnancy after ICSI had fewer chromatin condensation defects than did males who did not achieve any pregnancy (15.8 ± 12.0% vs. 11.4 ± 7.9%, respectively, P = 0.0242), which remained significant in multivariate regression analysis (RR = 0.40 [0.18 to 0.86], P = 0.02). RVA, DNA fragmentation, and aneuploidy were not predictive factors of ICSI outcomes. The pregnancy rate was significantly decreased by number of progressive motile spermatozoa with normal morphology after migration (P = 0.04). In female partners, 17β estradiol of less than 2000 pg/mL on the day of ovulation induction significantly reduced the occurrence of clinical pregnancy (P = 0.04). CONCLUSION: Sperm chromatin condensation defects were more frequently observed in couples with ICSI failure and should be considered a negative predictive factor for the occurrence of clinical pregnancy.
Authors: D P Evenson; L K Jost; D Marshall; M J Zinaman; E Clegg; K Purvis; P de Angelis; O P Claussen Journal: Hum Reprod Date: 1999-04 Impact factor: 6.918
Authors: J Auger; F Eustache; A G Andersen; D S Irvine; N Jørgensen; N E Skakkebaek; J Suominen; J Toppari; M Vierula; P Jouannet Journal: Hum Reprod Date: 2001-12 Impact factor: 6.918
Authors: Kjersten L Larson-Cook; John D Brannian; Keith A Hansen; Kay M Kasperson; Edward T Aamold; Donald P Evenson Journal: Fertil Steril Date: 2003-10 Impact factor: 7.329
Authors: Ramadan A Saleh; Ashok Agarwal; Essam A Nada; Mohamed H El-Tonsy; Rakesh K Sharma; Andrew Meyer; David R Nelson; Anthony J Thomas Journal: Fertil Steril Date: 2003-06 Impact factor: 7.329