| Literature DB >> 312886 |
K Meretey, U Böhm, A Falus, S Bozsoky.
Abstract
The presence of IgE in immune complex-enriched fractions prepared by 3% polyethylene glycol (PEG) precipitation was studied. The dissolved PEG-insoluble fraction was incubated with [125I]anti-IgE antibodies an then precipitated again with 3% PEG. Radioactivity in the washed pellets was taken as indicating IgE present in immune complex form. A calibration curve was constructed using artificially prepared IgE-anti-IgE complexes. The method developed may be useful to detect other components present in immune complex fractions.Entities:
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Year: 1979 PMID： 312886 DOI： 10.1016/0022-1759(79)90247-3
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303